Sequence analysis of fusion protein gene of Newcastle disease virus isolated from outbreaks in Egypt during 2006

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dc.contributor.authorMohamed, Mahmoud HA
dc.contributor.authorKumar, Sachin
dc.contributor.authorPaldurai, Anandan
dc.contributor.authorSamal, Siba K
dc.date.accessioned2013-01-10T20:54:39Z
dc.date.available2013-01-10T20:54:39Z
dc.date.issued2011-05-18
dc.description.abstractBackground: Newcastle disease virus represents APMV-1 and is the most characterized among all APMV types. The F protein cleavage site sequence is a well-characterized determinant of NDV pathogenicity in chickens. In this study, the sequences of fusion protein (F) gene of three Newcastle disease virus (NDV) strains isolated from outbreak in chickens in the Al-Sharkia province of Egypt in 2006 were determined. Findings: The viral genomic RNAs were extracted from the infective allantoic fluid and F gene is amplified using primer sets designed from the available sequences of NDV strains from GenBank. The pathogenicity of NDV strains was determined by three internationally recognized tests mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index. The phylogenetic analysis showed that the Egypt isolates are closely related with the genotype II of class II NDV strains. Conclusions: The sequences of the F genes of the 2006 Egypt isolates are closely related to that of the 2005 Egypt isolate from the same province suggesting that these strains are probably circulating in the vaccinated bird population in Egypt until development of an outbreak.en_US
dc.description.urihttps://doi.org/10.1186/1743-422X-8-237
dc.identifier.citationMohamed, M.H., Kumar, S., Paldurai, A. et al. Sequence analysis of fusion protein gene of Newcastle disease virus isolated from outbreaks in Egypt during 2006. Virol J 8, 237 (2011).en_US
dc.identifier.urihttp://hdl.handle.net/1903/13355
dc.language.isoen_USen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectNewcastle disease virusen_US
dc.subjectAfrican strainen_US
dc.subjectChickensen_US
dc.subjectSequenceen_US
dc.titleSequence analysis of fusion protein gene of Newcastle disease virus isolated from outbreaks in Egypt during 2006en_US
dc.typeArticleen_US

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