Developing Tools for Investigating Chemotaxis Signal Clusters in Bacillus subtilis

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dc.contributor.advisorStewart, Richard Cen_US
dc.contributor.authorRogers, James Allenen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2013-02-06T07:23:56Z
dc.date.available2013-02-06T07:23:56Z
dc.date.issued2012en_US
dc.description.abstractMany bacteria make use of a set of dedicated chemoreceptor proteins to control a His-Asp signaling system; this control converts environmental sensory information into instructions that regulate flagellar rotation, enabling chemotaxis. This thesis summarizes my investigations of some of the chemotaxis signaling proteins in <italic>Bacillus subtilis</italic>, particularly coupling proteins CheW and CheV. Proteins CheA, CheW, CheV, CheY, and FliM were each expressed in <italic>B. subtilis</italic> as translational fusions with either YFP or CFP. These fusion proteins were then shown to fluoresce in living bacterial cells. Motility experiments were conducted to compare the function of these fusion proteins to their wild type counterparts. This thesis proposes a series of experiments that would use these fluorescent fusion proteins to further explore the idea that these chemotaxis proteins change position when <italic>B. subtilis</italic> encounters chemostimuli.en_US
dc.identifier.urihttp://hdl.handle.net/1903/13567
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolledbacillus subtilisen_US
dc.subject.pquncontrolledchemotaxisen_US
dc.subject.pquncontrolledfluorescenceen_US
dc.subject.pquncontrolledsignal transductionen_US
dc.subject.pquncontrolledswimmingen_US
dc.subject.pquncontrolledtranslational fusionen_US
dc.titleDeveloping Tools for Investigating Chemotaxis Signal Clusters in Bacillus subtilisen_US
dc.typeThesisen_US

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