Integrated CMOS Capacitance Sensor And Microactuator Control Circuits For On-Chip Cell Monitoring

Simple item page
dc.contributor.advisorAbshire, Pamelaen_US
dc.contributor.authorBangalore Prakash, Somashekaren_US
dc.contributor.departmentElectrical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2009-01-24T06:45:37Z
dc.date.available2009-01-24T06:45:37Z
dc.date.issued2008-10-07en_US
dc.description.abstract"Cell Clinics," CMOS/MEMS hybrid microsystems for on-chip investigation of biological cells, are currently being engineered for a broad spectrum of applications including olfactory sensing, pathogen detection, cytotoxicity screening and biocompatibility characterization. In support of this effort, this research makes two primary contributions towards designing the cell-based lab-on-a-chip systems. Firstly it develops CMOS capacitance sensors for characterizing cell-related properties including cell-surface attachment, cell health and growth. Assessing these properties is crucial to all kinds of cell applications. The CMOS sensors measure substrate coupling capacitances of anchorage-dependent cells cultured on-chip in a standard in vitro environment. The biophysical phenomenon underlying the capacitive behavior of cells is the counterionic polarization around the insulating cell bodies when exposed to weak, low frequency electric fields. The measured capacitance depends on a variety of factors related to the cell, its growth environment and the supporting substrate. These include membrane integrity, morphology, adhesion strength and substrate proximity. The demonstrated integrated cell sensing technique is non-invasive, easy-to-use and offers the unique advantage of automated real time cell monitoring without the need for disruptive external forces or biochemical labeling. On top of the silicon-based cell sensing platform, the cell clinics microsystem comprises MEMS structures forming an array of lidded microvials for confining single cells or small cell groups within controllable microenvironments in close proximity to the sensor sites. The opening and closing of the microvial lids are controlled by actuator hinges employing an electroactive polymer material that can electrochemically actuate. In macro-scale setups such electrochemical actuation reactions are controlled by an electronic instrument called potentiostat. In order to enable system miniaturization and enhance portability of cell clinics, this research makes its second contribution by implementing and demonstrating a CMOS potentiostat module for in situ control of the MEMS actuators.en_US
dc.format.extent4624102 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/8781
dc.language.isoen_US
dc.subject.pqcontrolledEngineering, Electronics and Electricalen_US
dc.subject.pqcontrolledEngineering, Biomedicalen_US
dc.subject.pqcontrolledEngineering, Electronics and Electricalen_US
dc.subject.pquncontrolledbiological cellsen_US
dc.subject.pquncontrolledbiomedical transducersen_US
dc.subject.pquncontrolledcapacitance measurementen_US
dc.subject.pquncontrolledcapacitance transducersen_US
dc.subject.pquncontrolledCMOS integrated circuitsen_US
dc.subject.pquncontrolledmixed analog-digital integrated circuitsen_US
dc.titleIntegrated CMOS Capacitance Sensor And Microactuator Control Circuits For On-Chip Cell Monitoringen_US
dc.typeDissertationen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
umi-umd-5800.pdf
Size:
4.41 MB
Format:
Adobe Portable Document Format
Download

Collections

UMD Theses and Dissertations
Electrical & Computer Engineering Theses and Dissertations