ENZYME INHIBITION IN MICROFLUIDICS FOR RE-ENGINEERING BACTERIAL SYNTHESIS PATHWAYS

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dc.contributor.advisorRUBLOFF, GARY Wen_US
dc.contributor.authorLARIOS BERLIN, DEAN EDWARDen_US
dc.contributor.departmentBioengineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2009-07-03T05:52:44Z
dc.date.available2009-07-03T05:52:44Z
dc.date.issued2009en_US
dc.description.abstractEnzyme-functionalized biological microfluidic (EF-BioMEMS) systems are an emerging class of lab-on-chip devices that manipulate enzymatic pathways by localizing reaction sites in a microfluidic network. An EF-BioMEM system was fabricated to demonstrate biochemical enzyme inhibition. Further, design optimizations to the EF-BioMEM system have been proposed which improve system sensitivity and performance. The <italic>pfs</italic> enzyme is part of the quorum-sensing pathway that ultimately produces the bacterial signaling molecule AI-2. An EF-BioMEM system was fabricated to investigate the <italic>pfs</italic> conversion activity in the presence of a transition state analogue inhibitor. A reduction in enzyme conversion was measured in microfluidics for increasing inhibitor concentration that was comparable to the response expected on a larger scale. This EF-BioMEMS testbed is capable of investigating other compounds that inhibit quorum sensing. Design improvements were demonstrates that improve overall system responsiveness by minimizing unintended reactions from non-specifically bound enzyme. EF-BioMEMS signal-to-background performance increased from 0.72 to 2.43.en_US
dc.format.extent4337420 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/9390
dc.language.isoen_US
dc.subject.pqcontrolledEngineering, Biomedicalen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pqcontrolledEngineering, Materials Scienceen_US
dc.subject.pquncontrolledbiomemsen_US
dc.subject.pquncontrolledchitosanen_US
dc.subject.pquncontrolledelectrodepositionen_US
dc.subject.pquncontrolledenzymeen_US
dc.subject.pquncontrolledinhibitionen_US
dc.subject.pquncontrolledmicrofluidicsen_US
dc.titleENZYME INHIBITION IN MICROFLUIDICS FOR RE-ENGINEERING BACTERIAL SYNTHESIS PATHWAYSen_US
dc.typeThesisen_US

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