DEVELOPMENT, VALIDATION, AND APPLICATION OF A QUANTITATIVE POLYMERASE CHAIN REACTION ASSAY TO ASSESS HEMATODINIUM PEREZI PREVALENCE IN ENVIRONMENTAL SAMPLES

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dc.contributor.advisorSchott, Eric Jen_US
dc.contributor.advisorJagus, Rosemaryen_US
dc.contributor.authorHanif, Ammaren_US
dc.contributor.departmentMarine-Estuarine-Environmental Sciencesen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2013-02-07T07:21:40Z
dc.date.available2013-02-07T07:21:40Z
dc.date.issued2012en_US
dc.description.abstractDinoflagellate parasites in the Hematodinium genus have emerged as important pathogens of economically important crustaceans worldwide, causing significant economic losses to fisheries and aquaculture. An understanding of the routes of infection in blue crab (Callinectes sapidus) populations would be facilitated by an improved knowledge of environmental reservoirs. A previously used PCR assay, based on small subunit rRNA sequences, lacked the specificity needed for Hematodinium perezi detection of environmental samples. Therefore a quantitative PCR assay based on the internal transcribed spacer 2 (ITS2) region of H. perezi rRNA genes was developed, validated, and applied to examine temporal and spatial incidences of environmental reservoirs in Delmarva coastal bays. H. perezi was detected in sediment and water in several Delmarva coastal bays, as well as the host, C. sapidus. Results suggest the existence of localized sediment reservoirs in areas where hydrological and geophysical features allow for the formation of cell deposits.en_US
dc.identifier.urihttp://hdl.handle.net/1903/13654
dc.subject.pqcontrolledParasitologyen_US
dc.subject.pqcontrolledEnvironmental studiesen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolledCallinectes sapidusen_US
dc.subject.pquncontrolledEnvironmenten_US
dc.subject.pquncontrolledHematodinium perezien_US
dc.subject.pquncontrolledITS2en_US
dc.subject.pquncontrolledParasiteen_US
dc.subject.pquncontrolledqPCRen_US
dc.titleDEVELOPMENT, VALIDATION, AND APPLICATION OF A QUANTITATIVE POLYMERASE CHAIN REACTION ASSAY TO ASSESS HEMATODINIUM PEREZI PREVALENCE IN ENVIRONMENTAL SAMPLESen_US
dc.typeThesisen_US

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