Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies

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dc.contributor.authorOlenginski, Lukasz T.
dc.contributor.authorTaiwo, Kehinde M.
dc.contributor.authorLeBlanc, Regan M.
dc.contributor.authorDayie, Theodore K.
dc.date.accessioned2023-10-30T17:48:02Z
dc.date.available2023-10-30T17:48:02Z
dc.date.issued2021-09-14
dc.description.abstractRNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution. Still, RNA analysis by NMR suffers from spectral overlap and line broadening, both of which worsen for larger RNAs. Incorporation of stable isotope labels into RNA has provided several solutions to these challenges. In this review, we summarize the benefits and limitations of various methods used to obtain isotope-labeled RNA building blocks and how they are used to prepare isotope-labeled RNA for NMR structure and dynamics studies.
dc.description.urihttps://doi.org/10.3390/molecules26185581
dc.identifierhttps://doi.org/10.13016/dspace/l2hc-csix
dc.identifier.citationOlenginski, L.T.; Taiwo, K.M.; LeBlanc, R.M.; Dayie, T.K. Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies. Molecules 2021, 26, 5581.
dc.identifier.urihttp://hdl.handle.net/1903/31195
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtChemistry & Biochemistryen_us
dc.relation.isAvailableAtCollege of Computer, Mathematical & Natural Sciencesen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectstable isotope
dc.subjectenzymes
dc.subjectnucleotides
dc.subjectNMR spectroscopy
dc.subjectRNA
dc.subjectstructure
dc.subjectdynamics
dc.titleIsotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies
dc.typeArticle
local.equitableAccessSubmissionNo

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