ISOTOPIC LABELING STRATEGIES AND NMR METHODOLOGIES TO FACILITATE RNA STRUCTURAL AND DYNAMICS STUDIES: APPLICATIONS TO A LONG NON-CODING RNA FROM KAPOSI’S SARCOMA-ASSOCIATED HERPESVIRUS

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dc.contributor.advisorDayie, Kwaku Ten_US
dc.contributor.authorBecette, Owenen_US
dc.contributor.departmentBiochemistryen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2020-07-17T05:30:12Z
dc.date.available2020-07-17T05:30:12Z
dc.date.issued2020en_US
dc.description.abstractRNAs are essential components of biological pathways that result in human disease making them attractive therapeutic targets. Currently, NMR spectroscopy is the only high-resolution technique capable of probing RNA interactions in solution. Although NMR spectroscopy is well-suited to characterize macromolecular interactions at atomic-level detail, the currently available isotopic labeling strategies and NMR methodologies are limited to relatively small RNAs (~ 30 nts, ~ 10 kDa). This size limitation is due to poor sensitivity and limited spectral resolution both of which worsen with increasing size. Here I present novel isotopic labeling schemes and NMR experiments to help expand the size limitations of NMR. These new technologies are then applied to characterize the structure and dynamics of a non-coding RNA from Kaposi’s sarcoma-associated herpesvirus (KSHV) that causes cancer in AIDS patients.en_US
dc.identifierhttps://doi.org/10.13016/rof7-ghhy
dc.identifier.urihttp://hdl.handle.net/1903/26334
dc.language.isoenen_US
dc.subject.pqcontrolledBiochemistryen_US
dc.subject.pquncontrolledchemo-enzymatic synthesisen_US
dc.subject.pquncontrolledfluorineen_US
dc.subject.pquncontrolledHBVen_US
dc.subject.pquncontrolledKSHVen_US
dc.subject.pquncontrolledNMRen_US
dc.subject.pquncontrolledRNAen_US
dc.titleISOTOPIC LABELING STRATEGIES AND NMR METHODOLOGIES TO FACILITATE RNA STRUCTURAL AND DYNAMICS STUDIES: APPLICATIONS TO A LONG NON-CODING RNA FROM KAPOSI’S SARCOMA-ASSOCIATED HERPESVIRUSen_US
dc.typeDissertationen_US

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