Isotope-Assisted Metabolic Flux Analysis: A Powerful Technique to Gain New Insights into the Human Metabolome in Health and Disease

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dc.contributor.authorMoiz, Bilal
dc.contributor.authorLi, Andrew
dc.contributor.authorPadmanabhan, Surya
dc.contributor.authorSriram, Ganesh
dc.contributor.authorClyne, Alisa Morss
dc.date.accessioned2023-10-17T14:49:44Z
dc.date.available2023-10-17T14:49:44Z
dc.date.issued2022-11-04
dc.description.abstractCell metabolism represents the coordinated changes in genes, proteins, and metabolites that occur in health and disease. The metabolic fluxome, which includes both intracellular and extracellular metabolic reaction rates (fluxes), therefore provides a powerful, integrated description of cellular phenotype. However, intracellular fluxes cannot be directly measured. Instead, flux quantification requires sophisticated mathematical and computational analysis of data from isotope labeling experiments. In this review, we describe isotope-assisted metabolic flux analysis (iMFA), a rigorous computational approach to fluxome quantification that integrates metabolic network models and experimental data to generate quantitative metabolic flux maps. We highlight practical considerations for implementing iMFA in mammalian models, as well as iMFA applications in in vitro and in vivo studies of physiology and disease. Finally, we identify promising new frontiers in iMFA which may enable us to fully unlock the potential of iMFA in biomedical research.
dc.description.urihttps://doi.org/10.3390/metabo12111066
dc.identifierhttps://doi.org/10.13016/dspace/ttgw-j7as
dc.identifier.citationMoiz, B.; Li, A.; Padmanabhan, S.; Sriram, G.; Clyne, A.M. Isotope-Assisted Metabolic Flux Analysis: A Powerful Technique to Gain New Insights into the Human Metabolome in Health and Disease. Metabolites 2022, 12, 1066.
dc.identifier.urihttp://hdl.handle.net/1903/31036
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtA. James Clark School of Engineeringen_us
dc.relation.isAvailableAtFischell Department of Bioengineeringen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectmetabolic flux analysis
dc.subjectfluxomics
dc.subjectisotopic non-stationary metabolic flux analysis
dc.subjectmetabolic engineering
dc.subjectmetabolomics
dc.subjectmass sprectpmetry
dc.subjectNMR
dc.titleIsotope-Assisted Metabolic Flux Analysis: A Powerful Technique to Gain New Insights into the Human Metabolome in Health and Disease
dc.typeArticle
local.equitableAccessSubmissionNo

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