Lab-on-CMOS Sensors and Real-time Imaging for Biological Cell Monitoring

Senevirathna, Bathiya

Lab-on-CMOS Sensors and Real-time Imaging for Biological Cell Monitoring

dc.contributor.advisor	Abshire, Pamela	en_US
dc.contributor.author	Senevirathna, Bathiya	en_US
dc.contributor.department	Electrical Engineering	en_US
dc.contributor.publisher	Digital Repository at the University of Maryland	en_US
dc.contributor.publisher	University of Maryland (College Park, Md.)	en_US
dc.date.accessioned	2019-10-01T05:38:54Z
dc.date.available	2019-10-01T05:38:54Z
dc.date.issued	2019	en_US
dc.description.abstract	Monitoring biological cell growth and viability is essential for in vivo biomedical diagnosis and therapy, and in vitro studies of pharmaceutical efficacy and material toxicity. Conventional monitoring techniques involve the use of dyes and markers that can potentially introduce side effects into the cell culture and often function as end-point assays. This eliminates the opportunity to track fast changes and to determine temporal correlation between measurements. Particularly in drug screening applications, high-temporal resolution cell viability data could inform decisions on drug application protocols that could lead to better treatment outcomes. This work presents development of a lab-on-chip (LoC) sensor for real-time monitoring of biological cell viability and proliferation, to provide a comprehensive picture of the changes cells undergo during their lifecycle. The LoC sensor consists of a complementary metal-oxide-semiconductor (CMOS) chip that measures the cell-to-substrate coupling of adherent cells that are cultured directly on top. This technique is non-invasive, does not require biochemical labeling, and allows for automated and unsupervised cell monitoring. The CMOS capacitance sensor was designed to addresses the ubiquitous challenges of sensitivity, noise coupling, and dynamic range that affect existing sensors. The design includes on-chip digitization, serial data output, and programmable control logic in order to facilitate packaging requirements for biological experiments. Only a microcontroller is required for readout, making it suitable for applications outside the traditional laboratory setting. An imaging platform was developed to provide time-lapse images of the sensor surface, which allowed for concurrent visual and capacitance observation of the cells. Results showed the ability of the LoC sensor to detect single cell binding events and changes in cell morphology. The sensor was used in in vitro experiments to monitor chemotherapeutic agent potency on drug-resistant and drug-sensitive cancer cell lines. Concentrations higher than 5 μM elicited cytotoxic effects on both cell lines, while a dose of 1 μM allowed discrimination of the two cell types. The system demonstrates the use of real-time capacitance measurements as a proof-of-concept tool that has potential to hasten the drug development process.	en_US
dc.identifier	https://doi.org/10.13016/nt89-2s5k
dc.identifier.uri	http://hdl.handle.net/1903/25133
dc.language.iso	en	en_US
dc.subject.pqcontrolled	Electrical engineering	en_US
dc.subject.pqcontrolled	Biomedical engineering	en_US
dc.subject.pquncontrolled	biosensor	en_US
dc.subject.pquncontrolled	cell viability	en_US
dc.subject.pquncontrolled	lab on a chip	en_US
dc.subject.pquncontrolled	real-time imaging	en_US
dc.subject.pquncontrolled	very-large-scale integration	en_US
dc.title	Lab-on-CMOS Sensors and Real-time Imaging for Biological Cell Monitoring	en_US
dc.type	Dissertation	en_US