Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Qβ Conjugates as a Vaccine Candidate

dc.contributor.authorZong, Guanghui
dc.contributor.authorToonstra, Christian
dc.contributor.authorYang, Qiang
dc.contributor.authorZhang, Roushu
dc.contributor.authorWang, Lai-Xi
dc.date.accessioned2023-10-27T18:25:17Z
dc.date.available2023-10-27T18:25:17Z
dc.date.issued2021-11-21
dc.description.abstractThe broadly neutralizing antibody PG9 recognizes a unique glycopeptide epitope in the V1V2 domain of HIV-1 gp120 envelope glycoprotein. The present study describes the design, synthesis, and antibody-binding analysis of HIV-1 V1V2 glycopeptide-Qβ conjugates as a mimic of the proposed neutralizing epitope of PG9. The glycopeptides were synthesized using a highly efficient chemoenzymatic method. The alkyne-tagged glycopeptides were then conjugated to the recombinant bacteriophage (Qβ), a virus-like nanoparticle, through a click reaction. Antibody-binding analysis indicated that the synthetic glycoconjugates showed significantly enhanced affinity for antibody PG9 compared with the monomeric glycopeptides. It was also shown that the affinity of the Qβ-conjugates for antibody PG9 was dependent on the density of the glycopeptide antigen display. The glycopeptide-Qβ conjugates synthesized represent a promising candidate of HIV-1 vaccine.
dc.description.urihttps://doi.org/10.3390/ijms222212538
dc.identifierhttps://doi.org/10.13016/dspace/ru7s-b2de
dc.identifier.citationZong, G.; Toonstra, C.; Yang, Q.; Zhang, R.; Wang, L.-X. Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Qβ Conjugates as a Vaccine Candidate. Int. J. Mol. Sci. 2021, 22, 12538.
dc.identifier.urihttp://hdl.handle.net/1903/31165
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtChemistry & Biochemistryen_us
dc.relation.isAvailableAtCollege of Computer, Mathematical & Natural Sciencesen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectglycopeptide
dc.subjectglycoconjugate
dc.subjectneutralizing epitope
dc.subjectbacteriophage Qβ
dc.subjectneutralizing antibody
dc.subjectHIV vaccine
dc.titleChemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Qβ Conjugates as a Vaccine Candidate
dc.typeArticle
local.equitableAccessSubmissionNo

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