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Title: Association between increased hepatic lipid storage and impaired hepatic mitochondrial function in ovariectomized mice
Authors: Valencia, Ana Patricia
Advisors: Spangenburg, Espen E
Department/Program: Kinesiology
Type: Thesis
Sponsors: Digital Repository at the University of Maryland
University of Maryland (College Park, Md.)
Subjects: Physiology
Health sciences
Cellular biology
Keywords: fatty liver
Hepatic steatosis
reactive oxygen species
Issue Date: 2012
Abstract: Reduced ovarian function is associated with development of the metabolic syndrome (MetS). Increased risk for MetS is strongly linked to hepatic metabolic dysfunction. However, at this time few studies have examined metabolic function of hepatic tissue under conditions of reduced ovarian function. The purpose of this study was to determine if ovariectomy (OVX) impaired hepatic mitochondrial function and its potential association with sirtuin (SIRT) function. Female C57BL/6 mice were divided into two groups (SHAM, OVX). Hepatic mitochondrial function was measured by assessing oxygen consumption, reactive oxygen species (ROS) production, and mitochondrial protein content. In addition, mitochondrial acetylation status and SIRT protein content was determined. The OVX group exhibited increased ROS production compared to the SHAM group. However, no differences were detected in oxygen consumption, mitochondrial protein content, acetylation status, or total SIRT content between groups. The data shows that ovariectomy increases mitochondrial ROS production, which suggests a novel mechanism to consider.
Appears in Collections:Kinesiology Theses and Dissertations
UMD Theses and Dissertations

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Valencia_umd_0117N_13347.pdfRESTRICTED ACCESS2.22 MBAdobe PDF140View/Open
AnimalProtocol_Spangenburg_2010_R-10-40.pdfRESTRICTED ACCESS219.6 kBAdobe PDF116View/Open

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