Rudrappa, Shashidhara GovindareddyIn chickens, the primary energy substrate is lipid during embryogenesis and carbohydrate after hatch. Accordingly, chicks adapt their metabolism to utilize glucose after hatch; however, little is known about metabolic adaptation in developing lymphocytes. Therefore, the objective of this dissertation was to examine metabolic adaptation in developing lymphocytes and the associated impact on their development. The first objective examined energy substrate utilization in bursacytes and thymocytes during the embryonic to posthatch transition. Glucose metabolism increased in both lymphocyte populations during the first two weeks posthatch due to increased glucose transporter-3 mRNA abundance, glucose uptake and hexokinase activity. Additionally, some of these metabolic markers were positively correlated with the serum glucose concentration. Glutamine metabolism increased in bursacytes only, and lipid metabolism was unaltered in both populations. Collectively, glucose is a preferred energy substrate for lymphocytes posthatch, and glucose utilization by developing lymphocytes may be related to the serum glucose concentration. The second objective determined the effect of glucose availability on thymocyte metabolism, energy status and survival. Embryonic thymic lobes were grown in culture in media containing varying glucose concentrations. Thymocyte glucose metabolism and mitochondria membrane potential were highest in 15 mM glucose and apoptosis was highest in 5mM glucose. Collectively, glucose availability regulates glucose metabolism in thymocytes, and these changes in glucose metabolism were related to thymocyte energy status and survival. The third objective determined the effect of glucose availability on T cell development. Thymocyte Interleukin-7Rα (IL-7Rα) mRNA abundance and CD4+ T cell numbers over the culture period were dependent upon glucose availability. Between 12 and 24 h, thymocyte IL-7Rα mRNA abundance increased in 5 mM increased 1.74-fold, while it decreased in 15 mM by 58.6%. CD4+ numbers decreased with time in 5 mM, whereas they increased with time in 15 mM. T cell receptor (TCR) β excision circles were higher in 15 mM compared to 5 mM at 12 h. Glucose availability alters TCR β rearrangement, IL-7Rα gene expression and CD4+ T cell development, which may influence naïve T cell generation. As thymocytes develop in a low glucose environment in ovo, this may be one factor that limits T cell development until hatch.en-USDissertationAgriculture, Animal Culture and NutritionLymphocyteschickensenergyapoptosisposthatchglucose