INVESTIGATION BY MASS SPECTROMETRY OF THE UBIQUITOME AND PROTEIN CARGO OF EXOSOMES DERIVED FROM MYELOID-DERIVED SUPPRESSOR CELLS

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dc.contributor.advisorFenselau, Catherineen_US
dc.contributor.authorAdams, Katherine R.en_US
dc.contributor.departmentChemistryen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2016-09-08T05:40:24Z
dc.date.available2016-09-08T05:40:24Z
dc.date.issued2016en_US
dc.description.abstractExosomes released by myeloid-derived suppressor cells (MDSC) are 30 nm in diameter extracellular vesicles that have been shown to carry biologically active proteins as well as ubiquitin molecules. Ubiquitin is known to have many functions, including involvement in the formation of exosomes, although the exact role is highly contested. In the study reported here, the proteome and ubiquitome of MDSC exosomes has been investigated by bottom-up proteomics techniques. This report identifies more than 1000 proteins contained in the MDSC exosome cargo and 489 sites of ubiquitination in more than 300 ubiquitinated proteins based on recognition of glycinylglycine tagged peptides without antibody enrichment. This has allowed extensive chemical and biological characterization of the ubiquitinated cohort compared to that of the entire protein cargo to support hypotheses on the role of ubiquitin in exosomes.en_US
dc.identifierhttps://doi.org/10.13016/M2CV46
dc.identifier.urihttp://hdl.handle.net/1903/18763
dc.language.isoenen_US
dc.subject.pqcontrolledAnalytical chemistryen_US
dc.subject.pqcontrolledBiochemistryen_US
dc.subject.pquncontrolledExosomesen_US
dc.subject.pquncontrolledLC-MS/MSen_US
dc.subject.pquncontrolledMDSCen_US
dc.subject.pquncontrolledproteasomeen_US
dc.subject.pquncontrolledproteomeen_US
dc.subject.pquncontrolledubiquitomeen_US
dc.titleINVESTIGATION BY MASS SPECTROMETRY OF THE UBIQUITOME AND PROTEIN CARGO OF EXOSOMES DERIVED FROM MYELOID-DERIVED SUPPRESSOR CELLSen_US
dc.typeThesisen_US

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