THE ROLE OF EXCITATION-CONTRACTION COUPLING FAILURE IN MUSCLE FATIGUE AND WEAKNESS OF DYSTROPHIC SKELETAL MUSCLE

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dc.contributor.advisorChin, Eva Ren_US
dc.contributor.authorMazala, Davi Augusto Garciaen_US
dc.contributor.departmentKinesiologyen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2011-07-06T06:06:06Z
dc.date.available2011-07-06T06:06:06Z
dc.date.issued2011en_US
dc.description.abstractAlterations in intracellular calcium (Ca2+) are thought to play an important role in skeletal muscle weakness associated with muscular dystrophy due to the activation of Ca2+-regulated proteases (calpains). It was hypothesized that impairments in Ca2+ regulation are exacerbated in dystrophic muscle and that calpain inhibition could attenuate the muscle weakness induced by fatiguing contractions. Single muscle fibres from control and dystrophic mice lacking dystrophin (mdx) and utrophin plus dystrophin (Utr-/-/mdx) were used. Fibres from Utr-/-/mdx mice had similar peak tetanic Ca2+ compared to control and mdx mice, however Utr-/-/mdx mice took longer to clear the released Ca2+. All fibres showed similar time to fatigue but fewer mdx and Utr-/-/mdx fibres remained excitable 1hr after fatiguing contractions. Exposure to a calpain inhibitor improved Ca2+ levels in dystrophic fibres (mdx; trend only in Utr-/-/mdx) after fatigue. Together, these data indicate that calpains play a role in prolonged muscle weakness after fatiguing contractions.en_US
dc.identifier.urihttp://hdl.handle.net/1903/11600
dc.subject.pqcontrolledKinesiologyen_US
dc.subject.pqcontrolledMolecular Biologyen_US
dc.subject.pquncontrolledcalciumen_US
dc.subject.pquncontrolledcalpainsen_US
dc.subject.pquncontrolleddouble mutanten_US
dc.subject.pquncontrolledDuchenneen_US
dc.subject.pquncontrolledmdxen_US
dc.titleTHE ROLE OF EXCITATION-CONTRACTION COUPLING FAILURE IN MUSCLE FATIGUE AND WEAKNESS OF DYSTROPHIC SKELETAL MUSCLEen_US
dc.typeThesisen_US

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