Evaluation of Pv11 Cell Immobilization Techniques Across Substrates

Abstract

Avidin-biotin binding, Cell-Tak, and crosslinked alginate hydrogel were compared to collagen-BAM, our standard method, to assess how well they immobilized the cells and how the cells subsequently responded to odorants. These alternate methods exhibited high, although inconsistent, immobilization of cells, and the cells remained responsive to pentyl acetate, potentially offering new alternatives for Pv11 cell immobilization.