REFINING METAGENETIC ENVIRONMENTAL DNA TECHNIQUES FOR SENSITIVE BEE COMMUNITY MONITORING

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dc.contributor.advisorRichardson, Rodney Ten_US
dc.contributor.authorAvalos, Graceen_US
dc.contributor.departmentMarine-Estuarine-Environmental Sciencesen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2024-02-14T06:55:17Z
dc.date.available2024-02-14T06:55:17Z
dc.date.issued2023en_US
dc.description.abstractMolecular taxonomic detection is now widespread across the sciences, because of advances in direct PCR, improved marker selection, and increases in sequencing throughput. Facilitated by these advances in sequencing, methodological sensitivity of sample identification has improved substantially. Metagenetic techniques to infer what species are present in a sample by sequencing unknown samples and comparing them to known references has the potential to advance our understanding of biodiversity. Metagenetic analysis of environmental DNA (eDNA) represents a novel, non-lethal method for characterizing floral-associated arthropod communities. Diverse arthropod assemblages interact with flowers, and floral surfaces have been shown to harbor arthropod DNA. We performed metagenetic sequencing on eDNA isolated from flower samples and honey bee-collected pollen samples using multiple markers and compared the frequency and taxonomic breadth of eDNA detections across these genetic markers and substrate types. Understanding which markers and substrates are most effective for eDNA characterization of floral-associated arthropod communities will guide future research and enable low-risk detection of threatened or endangered arthropods.en_US
dc.identifierhttps://doi.org/10.13016/brwf-vbvm
dc.identifier.urihttp://hdl.handle.net/1903/31785
dc.language.isoenen_US
dc.subject.pqcontrolledEntomologyen_US
dc.subject.pqcontrolledEcologyen_US
dc.subject.pqcontrolledEnvironmental scienceen_US
dc.subject.pquncontrolledbee monitoringen_US
dc.subject.pquncontrolledcommunity ecologyen_US
dc.subject.pquncontrolledeDNAen_US
dc.subject.pquncontrolledfloral associationen_US
dc.subject.pquncontrolledpollinatoren_US
dc.subject.pquncontrolledpollinator networken_US
dc.titleREFINING METAGENETIC ENVIRONMENTAL DNA TECHNIQUES FOR SENSITIVE BEE COMMUNITY MONITORINGen_US
dc.typeThesisen_US

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