Differential Expression of CD45RO and CD45RA in Bovine T Cells

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dc.contributor.authorKandel, Anmol
dc.contributor.authorLi, Lei
dc.contributor.authorHada, Akanksha
dc.contributor.authorXiao, Zhengguo
dc.date.accessioned2023-10-25T16:14:13Z
dc.date.available2023-10-25T16:14:13Z
dc.date.issued2022-06-04
dc.description.abstractEffective vaccination induces immune memory to protect animals upon pathogen re-encounter. Despite contradictory reports, bovine memory T cells are identified based on two isoforms of CD45, expression of CD45RO plus exclusion of CD45RA. In this report, we contrasted CD45RA/RO expression on circulatory T cells with IFNγ and IL4 expression induced by a conventional method. To our surprise, 20% of cattle from an enclosed herd did not express CD45RO on T cells without any significant difference on CD45RA expression and IFNγ or IL4 induction. In CD45RO expressing cattle, CD45RA and CD45RO expressions excluded each other, with dominant CD45RO (>90%) expression on gamma delta (γδ) followed by CD4+ (60%) but significantly higher CD45RA expression on CD8+ T cells (about 80%). Importantly, more than 80% of CD45RO expressing CD4+ and CD8+ T cells failed to produce IFNγ and IL-4; however, within the cytokine inducing cells, CD4+ T cells highly expressed CD45RO but those within CD8+ T cells mostly expressed CD45RA. Hence, CD45RO is not ubiquitously expressed in cattle, and rather than with memory phenotype, CD45RA/RO expression are more associated with distinct T cell subtypes.
dc.description.urihttps://doi.org/10.3390/cells11111844
dc.identifierhttps://doi.org/10.13016/dspace/gfwz-auz4
dc.identifier.citationKandel, A.; Li, L.; Hada, A.; Xiao, Z. Differential Expression of CD45RO and CD45RA in Bovine T Cells. Cells 2022, 11, 1844.
dc.identifier.urihttp://hdl.handle.net/1903/31109
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtAnimal & Avian Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectcattle
dc.subjectmemory
dc.subjectCD4+ T cells
dc.subjectCD8+ T cells
dc.subjectγδ T cells
dc.subjectCD45RO
dc.subjectCD45RA
dc.subjectIFNγ
dc.subjectIL4
dc.subjectPBMCs
dc.titleDifferential Expression of CD45RO and CD45RA in Bovine T Cells
dc.typeArticle
local.equitableAccessSubmissionNo

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