Modification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching

dc.contributor.authorRhoads, Melissa K.
dc.contributor.authorHauk, Pricila
dc.contributor.authorGupta, Valerie
dc.contributor.authorBookstaver, Michelle L.
dc.contributor.authorStephens, Kristina
dc.contributor.authorPayne, Gregory F.
dc.contributor.authorBentley, William E.
dc.date.accessioned2023-11-28T15:04:19Z
dc.date.available2023-11-28T15:04:19Z
dc.date.issued2018-02-06
dc.description.abstractThis work sets out to provide a self-assembled biopolymer capsule activated with a multi-functional enzyme for localized delivery. This enzyme, SsoPox, which is a lactonase and phosphotriesterase, provides a means of interrupting bacterial communication pathways that have been shown to mediate pathogenicity. Here we demonstrate the capability to express, purify and attach SsoPox to the natural biopolymer chitosan, preserving its activity to “neutralize” long-chain autoinducer-1 (AI-1) communication molecules. Attachment is shown via non-specific binding and by engineering tyrosine and glutamine affinity ‘tags’ at the C-terminus for covalent linkage. Subsequent degradation of AI-1, in this case N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL), serves to “quench” bacterial quorum sensing (QS), silencing intraspecies communication. By attaching enzymes to pH-responsive chitosan that, in turn, can be assembled into various forms, we demonstrate device-based flexibility for enzyme delivery. Specifically, we have assembled quorum-quenching capsules consisting of an alginate inner core and an enzyme “decorated” chitosan shell that are shown to preclude bacterial QS crosstalk, minimizing QS mediated behaviors.
dc.description.urihttps://doi.org/10.3390/molecules23020341
dc.identifierhttps://doi.org/10.13016/dspace/pkms-i5ry
dc.identifier.citationRhoads, M.K.; Hauk, P.; Gupta, V.; Bookstaver, M.L.; Stephens, K.; Payne, G.F.; Bentley, W.E. Modification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching. Molecules 2018, 23, 341.
dc.identifier.urihttp://hdl.handle.net/1903/31485
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtA. James Clark School of Engineeringen_us
dc.relation.isAvailableAtFischell Department of Bioengineeringen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectquorum quenching
dc.subjectantibiotics
dc.subjectorganophosphate
dc.subjectenzyme attachment
dc.subjectenzyme delivery
dc.titleModification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching
dc.typeArticle
local.equitableAccessSubmissionNo

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