A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus

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dc.contributor.authorYang, Yonglin
dc.contributor.authorLin, Shaoli
dc.contributor.authorNan, Yuchen
dc.contributor.authorMa, Zexu
dc.contributor.authorYang, Liping
dc.contributor.authorZhang, Yanjin
dc.date.accessioned2023-11-30T20:49:00Z
dc.date.available2023-11-30T20:49:00Z
dc.date.issued2016-08-18
dc.description.abstractHepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology.
dc.description.urihttps://doi.org/10.3390/v8080227
dc.identifierhttps://doi.org/10.13016/dspace/bsaw-ft9y
dc.identifier.citationYang, Y.; Lin, S.; Nan, Y.; Ma, Z.; Yang, L.; Zhang, Y. A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus. Viruses 2016, 8, 227.
dc.identifier.urihttp://hdl.handle.net/1903/31522
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectHepatitis E virus
dc.subjectHEV
dc.subjectORF3
dc.subjectVP13
dc.subjectPXXP motif
dc.subjectlinear epitope
dc.subjectmonoclonal antibody
dc.titleA Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus
dc.typeArticle
local.equitableAccessSubmissionNo

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