A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus
dc.contributor.author | Yang, Yonglin | |
dc.contributor.author | Lin, Shaoli | |
dc.contributor.author | Nan, Yuchen | |
dc.contributor.author | Ma, Zexu | |
dc.contributor.author | Yang, Liping | |
dc.contributor.author | Zhang, Yanjin | |
dc.date.accessioned | 2023-11-30T20:49:00Z | |
dc.date.available | 2023-11-30T20:49:00Z | |
dc.date.issued | 2016-08-18 | |
dc.description.abstract | Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology. | |
dc.description.uri | https://doi.org/10.3390/v8080227 | |
dc.identifier | https://doi.org/10.13016/dspace/bsaw-ft9y | |
dc.identifier.citation | Yang, Y.; Lin, S.; Nan, Y.; Ma, Z.; Yang, L.; Zhang, Y. A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus. Viruses 2016, 8, 227. | |
dc.identifier.uri | http://hdl.handle.net/1903/31522 | |
dc.language.iso | en_US | |
dc.publisher | MDPI | |
dc.relation.isAvailableAt | College of Agriculture & Natural Resources | en_us |
dc.relation.isAvailableAt | Department of Veterinary Medicine | en_us |
dc.relation.isAvailableAt | Digital Repository at the University of Maryland | en_us |
dc.relation.isAvailableAt | University of Maryland (College Park, MD) | en_us |
dc.subject | Hepatitis E virus | |
dc.subject | HEV | |
dc.subject | ORF3 | |
dc.subject | VP13 | |
dc.subject | PXXP motif | |
dc.subject | linear epitope | |
dc.subject | monoclonal antibody | |
dc.title | A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus | |
dc.type | Article | |
local.equitableAccessSubmission | No |