Characterization and Expression of RUB1 Conjugase and Cullin1-like Genes in Poplar Associated with Vegetative Bud Development and Dormancy

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dc.contributor.advisorColeman, Gary Den_US
dc.contributor.authorSaritas-Yildirim, Banuen_US
dc.contributor.departmentPlant Science and Landscape Architecture (PSLA)en_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2006-06-14T06:14:59Z
dc.date.available2006-06-14T06:14:59Z
dc.date.issued2006-06-12en_US
dc.description.abstractDormancy is an adaptive trait that ensures survival of plants in adverse growth conditions. By using phylogenetical, expression, and protein analysis, RUB1 conjugase and Cullin1 were characterized in poplar dormancy. RUB1 conjugase and Cullin1 were annotated using sequence homology approach. RUB1 conjugase was expressed in apical buds during dormancy but not detected in those of etr1-1 expressing poplars. Its transcript abundance was reduced in axillary buds, leaves and bark of etr1-1 expressing poplars compared to wild types. RUB1 conjugase expression was not affected by AVG but reduced by 50µM ACC in apical buds of etr1-1 expressing poplars. Cullin1 and EBF expression did not display significant difference in most tissues except for the Cullin1 expression in middle and bottom leaves during short-day treatments in wild types. Two novel RUB1-conjugated proteins were detected during low temperature treatments. This study has built a foundation for further analysis of targeted protein degradation in dormancy.en_US
dc.format.extent81379268 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/3654
dc.language.isoen_US
dc.subject.pqcontrolledBiology, Molecularen_US
dc.subject.pqcontrolledBiology, Botanyen_US
dc.subject.pquncontrolledPopulusen_US
dc.subject.pquncontrolledDormancyen_US
dc.subject.pquncontrolledetr1-1en_US
dc.subject.pquncontrolledCullin1en_US
dc.subject.pquncontrolledRUB1 conjugaseen_US
dc.titleCharacterization and Expression of RUB1 Conjugase and Cullin1-like Genes in Poplar Associated with Vegetative Bud Development and Dormancyen_US
dc.typeThesisen_US

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