Pilot Study to Detect Genes Involved in DNA Damage and Cancer in Humans: Potential Biomarkers of Exposure to E-Cigarette Aerosols

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dc.contributor.authorHamad, Samera H.
dc.contributor.authorBrinkman, Marielle C.
dc.contributor.authorTsai, Yi-Hsuan
dc.contributor.authorMellouk, Namya
dc.contributor.authorCross, Kandice
dc.contributor.authorJaspers, Ilona
dc.contributor.authorClark, Pamela I.
dc.contributor.authorGranville, Courtney A.
dc.date.accessioned2023-11-02T19:49:34Z
dc.date.available2023-11-02T19:49:34Z
dc.date.issued2021-03-22
dc.description.abstractThere is a paucity of data on how gene expression enables identification of individuals who are at risk of exposure to carcinogens from e-cigarette (e-cig) vaping; and how human vaping behaviors modify these exposures. This pilot study aimed to identify genes regulated from acute exposure to e-cig using RT-qPCR. Three subjects (2M and 1F) made three visits to the lab (nTOT = 9 visits); buccal and blood samples were collected before and immediately after scripted vaping 20 puffs (nTOT = 18 samples); vaping topography data were collected in each session. Subjects used their own e-cig containing 50:50 propylene glycol (PG):vegetable glycerine (VG) +3–6 mg/mL nicotine. The tumor suppressor TP53 was significantly upregulated in buccal samples. TP53 expression was puff volume and flow rate dependent in both tissues. In blood, the significant downregulation of N-methylpurine DNA glycosylase (MPG), a base excision repair gene, was consistent across all subjects. In addition to DNA repair pathway, cell cycle and cancer pathways were the most enriched pathways in buccal and blood samples, respectively. This pilot study demonstrates that vaping 20 puffs significantly alters expression of TP53 in human tissues; vaping behavior is an important modifier of this response. A larger study is needed to confirm these relationships.
dc.description.urihttps://doi.org/10.3390/genes12030448
dc.identifierhttps://doi.org/10.13016/dspace/e8z9-jfkv
dc.identifier.citationHamad, S.H.; Brinkman, M.C.; Tsai, Y.-H.; Mellouk, N.; Cross, K.; Jaspers, I.; Clark, P.I.; Granville, C.A. Pilot Study to Detect Genes Involved in DNA Damage and Cancer in Humans: Potential Biomarkers of Exposure to E-Cigarette Aerosols. Genes 2021, 12, 448.
dc.identifier.urihttp://hdl.handle.net/1903/31266
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtUniversity of Maryland (College Park, Md)
dc.subjectDNA damage
dc.subjectgene expression
dc.subjectelectronic cigarettes
dc.subjecthuman buccal and blood
dc.subjectbiomarkers
dc.subjectvaping behaviors
dc.titlePilot Study to Detect Genes Involved in DNA Damage and Cancer in Humans: Potential Biomarkers of Exposure to E-Cigarette Aerosols
dc.typeArticle
local.equitableAccessSubmissionNo

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