The characterization of secretion and cytolytic functionality of Mycobacterium virulence factors ESAT-6 and CFP-10
| dc.contributor.advisor | Gao, Lian-Yong | en_US |
| dc.contributor.author | Manoranjan, Joanna Maya | en_US |
| dc.contributor.department | Cell Biology & Molecular Genetics | en_US |
| dc.contributor.publisher | Digital Repository at the University of Maryland | en_US |
| dc.contributor.publisher | University of Maryland (College Park, Md.) | en_US |
| dc.date.accessioned | 2007-02-01T20:24:06Z | |
| dc.date.available | 2007-02-01T20:24:06Z | |
| dc.date.issued | 2007-01-02 | en_US |
| dc.description.abstract | Mycobacterium tuberculosis, the causative agent for the Tuberculosis disease, contains a genetic virulence locus named Region of Difference 1 (RD1). RD1 encodes for a novel secretion system that exports two small, immunogenic proteins named ESAT-6 and CFP-10. Although these proteins are involved in M.tuberculosis pathogenesis, their function remains largely unknown. This work employs the use of M.marinum, a species genetically closely related to M.tuberculosis, to study the homologous RD1 region with a focus on the function of ESAT-6 and CFP-10. This also involves the characterization of individual RD1 genes in the secretion and stability of ESAT-6 and CFP-10. The RD1 locus has been implicated in playing a role in bacterial spreading through host cell lysis and necrotic granuloma formation- a hallmark of pathogenic mycobacterium infections. Consequently, this study establishes the putative role of ESAT-6 and CFP-10 in pathogenesis via an investigation into their cytolytic abilities against host macrophages and the bacteria's subsequent ability to spread during infection. | en_US |
| dc.format.extent | 737741 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/1903/4233 | |
| dc.language.iso | en_US | |
| dc.subject.pqcontrolled | Biology, Microbiology | en_US |
| dc.title | The characterization of secretion and cytolytic functionality of Mycobacterium virulence factors ESAT-6 and CFP-10 | en_US |
| dc.type | Thesis | en_US |
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