FUSARIUM SPECIES OF CUCUMIS MELO IN THE MID-ATLANTIC REGION OF THE US AND THEIR IMPACT ON SALMONELLA ENTERICA NEWPORT SURVIVAL AND INTERNALIZATION ON VARIOUS MELON CULTIVARS.

dc.contributor.advisorEverts, Dr. Kathryne Len_US
dc.contributor.advisorMicallef, Dr. Shirley Aen_US
dc.contributor.authorKorir, Robert Cheruiyoten_US
dc.contributor.departmentPlant Science and Landscape Architecture (PSLA)en_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2020-02-06T06:31:13Z
dc.date.available2020-02-06T06:31:13Z
dc.date.issued2019en_US
dc.description.abstractFruit rots caused by Fusarium spp. can lead to economic yield losses on melon (Cucumis melo). However, which Fusarium spp. are the most prevalent in Maryland and Delaware has not been documented. Several Salmonella enterica subsp. serovar Newport (S. Newport) outbreaks on melon have occurred over the past 25 years. Fusarium spp. infestation on melon have potential impact on survival and colonization of Salmonella. Our objectives were to identify Fusarium spp. infestations on melons within the Delmarva region, and evaluate their impact on survival and internalization of S. Newport on various melon cultivars. Fifty-six isolates were molecularly identified, according to Fusarium-ID online database, as Fusarium spp. (Fusarium fujikuroi-20, Fusarium proliferatum-18, Fusarium oxysporum-15, Fusarium graminearium-2, Fusarium verticilloides-1). Our findings revealed that most of the Fusarium isolates we collected were not pathogenic to melon fruit. We evaluated the impact of four Fusarium spp. (F. armeniacum, F. oxysporum, F. fujikuroi, and F. proliferatum) on S. Newport survival in five melon cultivars; ‘Arava’ (C. melo var. reticulatus, Galia), ‘Athena’ (var. reticulatus, muskmelon), ‘Dulce Nectar’ (var. inodorus, honeydew), ‘Jaune de Canaries’ (var. inodorus, Canary), and ‘Sivan’ (var. cantalupensis, Charentais). Impact of F. proliferatum on survival and interlization of S. Newport was evaluated on honeydew (smooth) and cantaloupe (netted) melons. Generally, Fusarium did not impact the survival of S. Newport, however greater survival of S. Newport was observed on the netted cultivars compared to the smooth surface melons. Fusarium fujikuroi significantly enhanced survival of Salmonella when inoculated on riper ‘Jaune de Canaries’ melons (above ¾ slip). However, when the experiments were replicated with less ripe (about ¾ slip) melon, F. fujikuroi did not significantly influence the growth of S. Newport. Salmonella Newport internalized in all treatments and the cantaloupe and honeydew melons, but variation in population levels were observed across the treatments. Overall, Fusarium proliferatum did not impact internalization of S. Newport on either melon type. This may be attributed to that Fusarium species used during this study were non-pathogenic. Salmonella Newport recovered gradually decreased with time. Fusarium species on melon, influence S. Newport colonization differently. Also, melon rind type affects the ability of S. Newport to survive and colonize differently.en_US
dc.identifierhttps://doi.org/10.13016/4tag-xqp9
dc.identifier.urihttp://hdl.handle.net/1903/25497
dc.language.isoenen_US
dc.subject.pqcontrolledPlant sciencesen_US
dc.subject.pqcontrolledPlant pathologyen_US
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pquncontrolledCultivarsen_US
dc.subject.pquncontrolledFusarium speciesen_US
dc.subject.pquncontrolledInteractionen_US
dc.subject.pquncontrolledMelonen_US
dc.subject.pquncontrolledSalmonella enterica Newporten_US
dc.subject.pquncontrolledSurvivalen_US
dc.titleFUSARIUM SPECIES OF CUCUMIS MELO IN THE MID-ATLANTIC REGION OF THE US AND THEIR IMPACT ON SALMONELLA ENTERICA NEWPORT SURVIVAL AND INTERNALIZATION ON VARIOUS MELON CULTIVARS.en_US
dc.typeDissertationen_US

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