Quantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program

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dc.contributor.authorGray, Kelsey M.
dc.contributor.authorJung, Jae W.
dc.contributor.authorInglut, Collin T.
dc.contributor.authorHuang, Huang-Chiao
dc.contributor.authorStroka, Kimberly M.
dc.date.accessioned2021-06-03T19:23:48Z
dc.date.available2021-06-03T19:23:48Z
dc.date.issued2020-02-11
dc.description.abstractThe endothelial cell–cell junctions of the blood–brain barrier (BBB) play a pivotal role in the barrier’s function. Altered cell–cell junctions can lead to barrier dysfunction and have been implicated in several diseases. Despite this, the driving forces regulating junctional protein presentation remain relatively understudied, largely due to the lack of efficient techniques to quantify their presentation at sites of cell–cell adhesion. Here, we used our novel Junction Analyzer Program (JAnaP) to quantify junction phenotype (i.e., continuous, punctate, or perpendicular) in response to various substrate compositions, cell culture times, and cAMP treatments in human brain microvascular endothelial cells (HBMECs). We then quantitatively correlated junction presentation with barrier permeability on both a “global” and “local” scale.en_US
dc.description.urihttps://doi.org/10.1186/s12987-020-0177-y
dc.identifierhttps://doi.org/10.13016/tbwp-hu6b
dc.identifier.citationGray, K.M., Jung, J.W., Inglut, C.T. et al. Quantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program. Fluids Barriers CNS 17, 16 (2020).en_US
dc.identifier.urihttp://hdl.handle.net/1903/27101
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtA. James Clark School of Engineeringen_us
dc.relation.isAvailableAtFischell Department of Bioengineeringen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectBlood–brain barrieren_US
dc.subjectcAMPen_US
dc.subjectJAnaPen_US
dc.subjectPermeabilityen_US
dc.titleQuantitatively relating brain endothelial cell–cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Programen_US
dc.typeArticleen_US

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