Implication of Matrix Metalloproteinases One and Two in Autophagic Cell Death in the Drosophila Salivary Gland

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dc.contributor.advisorBaehrecke, Eric Hen_US
dc.contributor.authorKobayashi, Marieen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2007-02-28T06:30:07Z
dc.date.available2007-02-28T06:30:07Z
dc.date.issued2006-12-06en_US
dc.description.abstractProgrammed cell death plays an important role in normal development. Defects in this process contribute to cancer. Matrix metalloproteinases (MMPs), zinc-dependant endopeptidases that cleave components of the extracellular matrix, are among the multiple protease types implicated in cell death. Here I provide evidence that MMPs function in <em>Drosophila</em> salivary gland cell death. Misexpression of the MMP inhibitor timp inhibited timely salivary gland cell death, while misexpression of mmp2 induced premature salivary gland cell death. mmp RNA interference was inconclusive because salivary gland persistence observed at 28°C was similar to fkh-GAL4 negative controls. MMPs and caspases might have an additive effect, since misexpression of timp and the caspase inhibitor p35 together enhanced salivary gland persistence compared to either timp or p35 misexpression alone. I also provide descriptive confocal microscopy of wild-type salivary glands using ?-Spectrin and the polarity marker Crumbs which suggest that polarity is lost during salivary gland cell death.en_US
dc.format.extent4358220 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/4303
dc.language.isoen_US
dc.subject.pqcontrolledBiology, Cellen_US
dc.subject.pqcontrolledBiology, Geneticsen_US
dc.subject.pquncontrolledMMPen_US
dc.subject.pquncontrolleddevelopmenten_US
dc.subject.pquncontrolledecdysoneen_US
dc.subject.pquncontrolledcaspaseen_US
dc.subject.pquncontrolledTIMPen_US
dc.subject.pquncontrolledautophagyen_US
dc.titleImplication of Matrix Metalloproteinases One and Two in Autophagic Cell Death in the Drosophila Salivary Glanden_US
dc.typeThesisen_US

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