Biochemical characterizations of extracellular vesicles shed by vegetative and sporulating Bacillus subtilis

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dc.contributor.advisorFenselau, Catherineen_US
dc.contributor.authorKim, Yejien_US
dc.contributor.departmentBiochemistryen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2015-09-18T05:38:39Z
dc.date.available2015-09-18T05:38:39Z
dc.date.issued2015en_US
dc.description.abstractSporulation of Bacilli is a developmental process that provides long-term viability in unfavorable environments. Recently, biogenesis of extracellular vesicles (EVs) from Bacilli has also been reported to participate in various physiological and pathogenic phenomena. In this study, EVs were isolated from vegetative and sporulating Bacillus subtilis cells and characterized using mass spectrometry (MS)-based proteomics, microscopy, and fluorescence spectrophotometry. The microscopic approach demonstrated that both vegetative and sporulating cells produce EVs. In the proteomic analysis, 156 proteins were identified with statistical significance in EVs collected at the vegetative phase and 185 proteins in EVs shed during sporulation. The two EV cargos showed qualitatively and quantitatively different proteome patterns. Sporulation-associated proteins had greater abundances in EVs at the sporulation stage. Additionally, a fusion-like event of EVs with B. subtilis cells was observed by a fluorescence de-quenching assay. Based on these observations, B. subtilis EVs are proposed to support intercellular communication and sporulation.en_US
dc.identifierhttps://doi.org/10.13016/M2PH01
dc.identifier.urihttp://hdl.handle.net/1903/16942
dc.language.isoenen_US
dc.subject.pqcontrolledBiochemistryen_US
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pqcontrolledBioinformaticsen_US
dc.subject.pquncontrolledAntibiotic activityen_US
dc.subject.pquncontrolledBacillus subtilisen_US
dc.subject.pquncontrolledBioinformaticsen_US
dc.subject.pquncontrolledExtracellular vesiclesen_US
dc.subject.pquncontrolledProteomicsen_US
dc.subject.pquncontrolledSporulationen_US
dc.titleBiochemical characterizations of extracellular vesicles shed by vegetative and sporulating Bacillus subtilisen_US
dc.typeThesisen_US

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