In vitro expansion of the mammary stem/progenitor cell population by xanthosine treatment

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dc.contributor.authorChoudhary, Ratan K
dc.contributor.authorCapuco, Anthony V
dc.date.accessioned2021-10-25T15:32:11Z
dc.date.available2021-10-25T15:32:11Z
dc.date.issued2012-06-14
dc.description.abstractMammary stem cells are critical for growth and maintenance of the mammary gland and therefore are of considerable interest for improving productivity and efficiency of dairy animals. Xanthosine treatment has been demonstrated to promote expansion of putative mammary stem cells in vivo, and hepatic and hair follicle stem cells in vitro. In the latter, xanthosine promoted the symmetrical division of hepatic and hair follicle stem cells. The objective of this study was to determine if treating primary cultures of bovine mammary epithelial cells (MEC) with xanthosine increases the stem/progenitor cell population by promoting symmetrical division of mammary stem cells. In vitro treatment with xanthosine increased the population of MEC during the exponential phase of cell growth, reducing the doubling time from 86 h in control cultures to 60 h in xanthosine-treated cultures. The bromodeoxyuridine (BrdU) labeling index and the proportion of MEC in S-phase both were increased by xanthosine treatment, indicating that increased cell accretion was due to increased cell proliferation. Analysis of daughter-pairs indicated that xanthosine promoted a shift from asymmetric to symmetric cell division. Moreover, the 30 % increase in symmetric cell division was concomitant with an increase in the proportion of MEC that were positive for a putative stem cell marker (FNDC3B) and a trend toward increased telomerase activity. These results suggest that xanthosine treatment in vitro can increase cell proliferation, promote symmetric cell division and enhance stem/progenitor cell activity. Xanthosine treatment increased the proliferation rate of bovine MEC in vitro. This was likely to be mediated by an increase in the proportion of stem/progenitor cells in the MEC population due to promotion of symmetrical stem cell division by xanthosine.en_US
dc.description.urihttps://doi.org/10.1186/1471-2121-13-14
dc.identifierhttps://doi.org/10.13016/a3ef-1zgk
dc.identifier.citationChoudhary, R.K., Capuco, A.V. In vitro expansion of the mammary stem/progenitor cell population by xanthosine treatment. BMC Cell Biol 13, 14 (2012).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28063
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtAnimal & Avian Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectMammary stem cellen_US
dc.subjectSelf renewalen_US
dc.subjectSymmetric divisionen_US
dc.subjectFNDC3Ben_US
dc.subjectTelomeraseen_US
dc.subjectBovineen_US
dc.titleIn vitro expansion of the mammary stem/progenitor cell population by xanthosine treatmenten_US
dc.typeArticleen_US

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