IDENTIFYING HIGHLY CONSERVED PATHOGENICITY GENES IN CHESTNUT BLIGHT AND POWDERY MILDEW FUNGI AS TARGETS FOR NOVEL FORMS OF HOST RESISTANCE

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dc.contributor.advisorXiao, Shunyuanen_US
dc.contributor.authorLevine, Bruce Jonathanen_US
dc.contributor.departmentPlant Science and Landscape Architecture (PSLA)en_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2019-06-20T05:39:00Z
dc.date.available2019-06-20T05:39:00Z
dc.date.issued2019en_US
dc.description.abstractA bioinformatic search of the genomes of chestnut blight fungus, Cryphonectria parasitica (Cp), and the Arabidopsis powdery mildew fungus, Golovinomyces cichoracearum (Gc), yielded six suspected pathogenicity genes with homologues in both species. Deletion of these genes by homologous gene replacement was attempted in Cp, with one success, TG4. The TG4-knockout strain showed changes in phenotype and reduced fungal virulence against chestnut. TG4 appears to be a promising target for host-induced gene silencing (HIGS) in transgenic American chestnut. The use of homologues from genetically tractable species like Cp can help overcome the obstacles to performing reverse genetics on intractable, biotrophic fungi such as Gc. Experiments underway involving the silencing and ectopic overexpression of the Gc homologues of the target genes provide a rapid method to study Cp genes, including to screen additional candidate genes as future targets for HIGS.en_US
dc.identifierhttps://doi.org/10.13016/9j2k-tmpv
dc.identifier.urihttp://hdl.handle.net/1903/22020
dc.language.isoenen_US
dc.subject.pqcontrolledPlant pathologyen_US
dc.subject.pqcontrolledPlant sciencesen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolledblighten_US
dc.subject.pquncontrolledchestnuten_US
dc.subject.pquncontrolledgenesen_US
dc.subject.pquncontrolledmildewen_US
dc.subject.pquncontrolledpowderyen_US
dc.subject.pquncontrolledresistanceen_US
dc.titleIDENTIFYING HIGHLY CONSERVED PATHOGENICITY GENES IN CHESTNUT BLIGHT AND POWDERY MILDEW FUNGI AS TARGETS FOR NOVEL FORMS OF HOST RESISTANCEen_US
dc.typeThesisen_US

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