ROLE OF ACID SPHINGOMYELINASE IN ICAM-1/NHE1-DEPENDENT ENDOCYTOSIS: IMPLICATIONS IN LEUKOCYTE TRANSMIGRATION

dc.contributor.advisorMuro, Silviaen_US
dc.contributor.authorSerrano, Danielen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2011-02-19T07:16:44Z
dc.date.available2011-02-19T07:16:44Z
dc.date.issued2010en_US
dc.description.abstractEngagement of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells (ECs) by anti-ICAM coated beads generates vesiculization via cell adhesion molecule (CAM)-mediated endocytosis, a clathrin-/caveolae-independent pathway involving Na+/H+ exchanger 1 (NHE1). ICAM-1 itself plays a key role in transendothelial migration (TEM) of leukocytes, particularly via the transcellular route. This involves endothelial endocytic vesicles that coalesce into transmigration pores, through which leukocytes transmigrate without disrupting EC junctions. The contribution of CAM-mediated endocytosis to the formation of docking sites and vesicular structures supporting TEM was explored in this study. Results show that the ICAM-1/NHE1-dependent CAM-mediated pathway associates with acid sphingomyelinase and ceramide. This supports plasmalemma deformability and cytoskeleton rearrangement, bridging these events to the formation of endothelial docking structures and vesicles involved in leukocyte transmigration.en_US
dc.identifier.urihttp://hdl.handle.net/1903/11278
dc.subject.pqcontrolledCellular Biologyen_US
dc.subject.pquncontrolledacid sphingomyelinaseen_US
dc.subject.pquncontrolledCAM-mediated endocytosisen_US
dc.subject.pquncontrolledICAM-1en_US
dc.subject.pquncontrolledleukocyte transmigrationen_US
dc.subject.pquncontrolledsphingomyelin/ceramide pathwayen_US
dc.subject.pquncontrolledtranscellularen_US
dc.titleROLE OF ACID SPHINGOMYELINASE IN ICAM-1/NHE1-DEPENDENT ENDOCYTOSIS: IMPLICATIONS IN LEUKOCYTE TRANSMIGRATIONen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Serrano_umd_0117N_11860.pdf
Size:
7.64 MB
Format:
Adobe Portable Document Format