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Calcium binding proteins and GAD immunoreactivity in the auditory system of Gekko Gecko

dc.contributor.advisorCarr, Catherine Een_US
dc.contributor.authorYan, Kaien_US
dc.description.abstractGeckos use vocalizations for intraspecific communication, but little is known about the organization of their central auditory system. We therefore used immunohistochemical techniques to delineate the auditory nuclei of the hindbrain, midbrain and thalamus. We used antibodies against the calcium binding proteins calretinin (CR), parvalbumin (PV), calbindin-D28k (CB) as well as antibodies against Glutamic Acid Decarboxylase (GAD) and synaptic vesicle protein 2 (SV2). Western blots showed that all five antibodies were specific within gecko brain. The fluorescence double labeling of calcium binding proteins yielded partly overlapping but mostly complementary distributions in the gecko auditory pathway. The differential expression of all calcium binding proteins and GAD, as well as SV2, characterized the structures of the gecko auditory system. The results indicate that the auditory structures and organization in Gekko gecko bear many similarities to the other land vertebrates.en_US
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dc.titleCalcium binding proteins and GAD immunoreactivity in the auditory system of Gekko Geckoen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.subject.pqcontrolledBiology, Neuroscienceen_US

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