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dc.contributor.advisorDevoe, Don Len_US
dc.contributor.authorZhou, Yien_US
dc.date.accessioned2007-09-28T15:03:25Z
dc.date.available2007-09-28T15:03:25Z
dc.date.issued2007-08-14en_US
dc.identifier.urihttp://hdl.handle.net/1903/7402
dc.description.abstractPolymer-based microfluidic systems have received considerable attention for high throughput chemical analysis. Recently, the ongoing development of microfluidics interfacing to high-accuracy mass spectrometry to identify large molecules had an important impact on biochemistry. A primary goal of this dissertation is the development of a microfluidic apparatus for performing microscale gel electrophoresis, coupled with integrated electrospray tips for either direct interfacing to mass spectrometry through ESI-MS, or coupling to MALDI-MS through the deposition of separated analyte onto a MALDI target for offline analysis. In this dissertation, microfabrication techniques for polymer-based microchip are developed. A novel electrospray interface is demonstrated with good performance. The optimization of multi-channel electrospray tips for multiplexed analysis from a single microfluidic chip was demonstrated. Gas-phased electrophoretic protein/peptide concentration on a pre-structured MALDI target was further demonstrated via theoretical and experimental analysis. The results for developing μGE-ES using linear polymer gel validate the underlined principles and specify challenges involved in coupling μGE to MS. Finally, cross-linked polyacrylamide gel was explored and characterized using in-situ photo- polymerization method in microchannels.en_US
dc.format.extent4254502 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.titleMICROFLUIDICS INTERFACING TO MASS SPECTROMETRYen_US
dc.typeDissertationen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentMechanical Engineeringen_US
dc.subject.pqcontrolledEngineering, Mechanicalen_US


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