Virtual reality is becoming mainstream in areas such as entertainment, medicine and training. However, the affect on a user’s perceived states are still to be fully understood. This study aims to add to the existing body of research by examining changes in user interfaces and the affect on perceived responses. Subjects in the study were exposed to two virtual environments, while undertaking a physical exercise task. Their perceived responses were captured through a combination of interviews, observations, and surveys. This differs from previous studies in that it is capturing the perceived differences between the environments themselves. The results highlighted that the content of the environments resulted in a variety of interesting, and unexpected, perceived responses.