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A NOVEL NON-LETHAL LAPAROSCOPIC APPROACH TO DETECT INTERSEX (TESTICULAR OOCYTES) IN LARGEMOUTH BASS (MICROPTERUS SALMOIDES) AND SMALLMOUTH BASS (MICROPTERUS DOLOMIEU)

dc.contributor.advisorYONKOS, LANCE Ten_US
dc.contributor.authorMacLeod, Alexander Hughen_US
dc.date.accessioned2016-09-07T05:30:17Z
dc.date.available2016-09-07T05:30:17Z
dc.date.issued2016en_US
dc.identifierhttps://doi.org/10.13016/M2M50Z
dc.identifier.urihttp://hdl.handle.net/1903/18623
dc.description.abstractThe appearance of testicular oocytes (TO) in wild fish populations has received considerable attention in the scientific literature and public media. Current methods to quantify TO are lethal; instead, a non-lethal alternative was examined. Laparoscopic insertion into the genital pore allowed internal visualization of the gonad and detection of TO by collecting five testis biopsies in smallmouth bass Micropterus dolomieu and largemouth bass Micropterus salmoides. Overall, biopsies quantified similar levels of TO detection and severity to conventional transverse sectioning with less than 10% mortality. Suitability of surgical anesthetics, tricaine methanesulfonate and electronarcosis were examined in laboratory and field applications. Electronarcosis had the added benefit of rapid sex identification and immediate release of female fish with minimal trauma, representing significant benefits when sampling small or compromised populations. Laparoscopy may be useful for monitoring the prevalence and severity of TO in these fish species when lethal sampling is not a desired outcome.en_US
dc.language.isoenen_US
dc.titleA NOVEL NON-LETHAL LAPAROSCOPIC APPROACH TO DETECT INTERSEX (TESTICULAR OOCYTES) IN LARGEMOUTH BASS (MICROPTERUS SALMOIDES) AND SMALLMOUTH BASS (MICROPTERUS DOLOMIEU)en_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentEnvironmental Science and Technologyen_US
dc.subject.pqcontrolledEnvironmental scienceen_US
dc.subject.pqcontrolledEndocrinologyen_US
dc.subject.pqcontrolledToxicologyen_US
dc.subject.pquncontrolledEndocrine Disruptionen_US
dc.subject.pquncontrolledIntersexen_US
dc.subject.pquncontrolledLaparoscopyen_US
dc.subject.pquncontrolledlargemouth and smallmouth bassen_US
dc.subject.pquncontrolledNon-lethalen_US
dc.subject.pquncontrolledTesticular Oocyteen_US


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