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Analysis of Flow-Based Microfluidic Gradient Generators for the Study of Bacterial Chemotaxis

dc.contributor.advisorRubloff, Gary Wen_US
dc.contributor.authorWolfram, Christopher Jamesen_US
dc.date.accessioned2015-06-25T05:41:51Z
dc.date.available2015-06-25T05:41:51Z
dc.date.issued2015en_US
dc.identifierhttps://doi.org/10.13016/M2790X
dc.identifier.urihttp://hdl.handle.net/1903/16455
dc.description.abstractChemotaxis is a phenomenon which enables cells to sense concentrations of certain chemical species in their microenvironment and move towards chemically favorable regions. This behavior is best understood in the bacteria Escherichia coli, which exhibits chemotaxis towards a variety of energy sources and signaling molecules. Recent advances in microbiology have engineered the chemotactic properties of bacteria to perform novel functions, but traditional methods of characterizing chemotaxis are not sufficient for such complex applications. The field of microfluidics offers solutions in the form of gradient generators. Many of these gradient generators are flow-based, where a chemical species diffuses across a solution moving through a microchannel. A microfluidic gradient generator was explored as a chemotaxis platform. Sources of error during experimental operation and methods of mitigating this error were demonstrated, and the fundamental theory behind these devices was examined. These devices were determined to be inadequate for the study of bacterial chemotaxis.en_US
dc.language.isoenen_US
dc.titleAnalysis of Flow-Based Microfluidic Gradient Generators for the Study of Bacterial Chemotaxisen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentMaterial Science and Engineeringen_US
dc.subject.pqcontrolledBiomedical engineeringen_US
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pquncontrolledbacteriaen_US
dc.subject.pquncontrolledchemotaxisen_US
dc.subject.pquncontrolledcolien_US
dc.subject.pquncontrolledflowen_US
dc.subject.pquncontrolledgradienten_US
dc.subject.pquncontrolledmicrofluidicen_US


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