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The Effect of Sialic Acid on the Success of Poultry Spermatozoa Cryopreservation

dc.contributor.advisorPorter, Tom Een_US
dc.contributor.advisorLong, Julie Aen_US
dc.contributor.authorTucker, Lisa Dianeen_US
dc.date.accessioned2014-06-26T05:41:49Z
dc.date.available2014-06-26T05:41:49Z
dc.date.issued2014en_US
dc.identifier.urihttp://hdl.handle.net/1903/15482
dc.description.abstractAvian semen cryopreservation is an invaluable reproductive strategy for conserving desirable genetic traits for commercial poultry production and endangered species conservation. Here we provide the effects of including sialic acid (SA) in the cryodiluent for tom and rooster sperm cryopreservation. Tom and rooster (n=6 males/treatment) semen was incubated at 25C or 4C with (0, 20, 40, 80, 120, 160, 200, or 240 ug/mL, depending on the species) SA for up to 2 h. At 30 min intervals, sperm viability and SA uptake were evaluated. Turkey and chicken (n=6 females/treatment) hens were inseminated with frozen/thawed semen treated with SA either pre-freeze only or pre-freeze/post-thaw (0, 20, 40, 80, 120, 160, or 240 ug/mL, depending on the species) and percent total fertility was evaluated. Our results demonstrate that the optimal SA incubation conditions for improved fertility with frozen/thawed sperm occurs with 160 ug/mL SA pre-freeze for turkey and 120 ug/mL SA pre-freeze for chicken (P<0.05). However, differences were observed between the two species with respect to the optimal temperature for SA incubation.en_US
dc.language.isoenen_US
dc.titleThe Effect of Sialic Acid on the Success of Poultry Spermatozoa Cryopreservationen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentAnimal Sciencesen_US
dc.subject.pqcontrolledAnimal sciencesen_US


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