University of Maryland LibrariesDigital Repository at the University of Maryland
    • Войти
    Просмотр элемента 
    •   Главная
    • College of Computer, Mathematical & Natural Sciences
    • Biology
    • Biology Research Works
    • Просмотр элемента
    •   Главная
    • College of Computer, Mathematical & Natural Sciences
    • Biology
    • Biology Research Works
    • Просмотр элемента
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Transpositionally active episomal hAT elements

    Thumbnail
    Открыть
    Stein, et al.pdf (819.3Kb)
    No. of downloads: 292

    Дата
    2009-12-14
    Автор
    O'Brochta, David A
    Stosic, Christina D
    Pilitt, Kristina
    Subramanian, Ramanand A
    Atkinson, Peter W
    Citation
    BMC Molecular Biology 2009, 10:108 doi:10.1186/1471-2199-10-108
    Metadata
    Показать полную информацию
    Аннотации
    Background: hAT elements and V(D)J recombination may have evolved from a common ancestral transposable element system. Extrachromosomal, circular forms of transposable elements(referred to here as episomal forms) have been reported yet their biological significance remains unknown. V(D)J signal joints, which resemble episomal transposable elements, have been considered non-recombinogenic products of V(D)J recombination and a safe way to dispose of excised chromosomal sequences. V(D)J signal joints can, however, participate in recombination reactions and the purpose of this study was to determine if hobo and Hermes episomal elements are also recombinogenic. Results: Up to 50% of hobo/Hermes episomes contained two intact, inverted-terminal repeats and 86% of these contained from 1-1000 bp of intercalary DNA. Episomal hobo/Hermes elements were recovered from Musca domestica (a natural host of Hermes), Drosophila melanogaster (a natural host of hobo) and transgenic Drosophila melanogaster and Aedes aegypti (with autonomous Hermes elements). Episomal Hermes elements were recovered from unfertilized eggs of M. domestica and D. melanogaster demonstrating their potential for extrachromosomal, maternal transmission. Reintegration of episomal Hermes elements was observed in vitro and in vivo and the presence of Hermes episomes resulted in lower rates of canonical Hermes transposition in vivo. Conclusion: Episomal hobo/Hermes elements are common products of element excision and can be maternally transmitted. Episomal forms of Hermes are capable of integration and also of influencing the transposition of canonical elements suggesting biological roles for these extrachromosomal elements in element transmission and regulation.
    URI
    http://hdl.handle.net/1903/13378
    Collections
    • Biology Research Works

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility
     

     

    Просмотр

    Весь DSpaceСообщества и коллекцииДата публикацииАвторыНазванияТематикаЭта коллекцияДата публикацииАвторыНазванияТематика

    Моя учетная запись

    ВойтиРегистрация
    Pages
    About DRUMAbout Download Statistics

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility