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Insight into the Structure and Mechanism of Iodotyrosine Deiodinase, the First Mammalian Member of the NADH Oxidase / Flavin Reductase Superfamily
(2006-07-18)
Iodotyrosine deiodinase (IYD) has remained poorly characterized for nearly 50 years in spite of its function as an iodine salvage pathway and its role in regulation of mammalian basal metabolism. IYD catalyzes the reductive ...
SCAFFOLDING-MEDIATED VIRUS ASSEMBLY: VISUALIZATION AND CHARACTERIZATION OF BACTERIOPHAGE T7 SCAFFOLDING PROTEIN
(2009)
In bacteriophage T7 as well as many other dsDNA phages and some animal viruses, scaffolding protein is essential for the accurate formation of the metastable precursor particle (prohead) and thus it is a vital aspect of ...
Location specific recognition of the recombination hot-spot "Chi" by the E. coli RecBCD enzyme
(2004-04-06)
RecBCD is an ATP-dependent helicase and exonuclease, which generates 3'-single-stranded DNA (ssDNA) ends used by RecA for homologous recombination. Critical to this function is its encounter with an regulatory DNA octamer ...
Top-Down Analysis of Bacterial Proteins by High-Resolution Mass Spectrometry
(2010)
In the biodefense and medical diagnostic fields, MALDI mass spectrometry-based systems are used for rapid characterization of microorganisms generally by detecting and discriminating the highly abundant protein mass-to-charge ...
Development and applications of codon scanning mutagenesis: A novel mutagenesis method that facilitates in-frame codon mutations
(2009)
The ability to create protein variants is a very valuable tool in biochemistry. Information about mechanistic roles of amino acid side chains, protein topology and binding can all be obtained. Methodologies to mutate ...
THERMODYNAMIC PROPERTIES OF THE UNFOLDED ENSEMBLE OF PROTEINS
(2010)
A random coil, whose size is determined by its excluded volume, and net energetic interactions with its environment, has served as a representation of the unfolded ensemble of proteins. The work in this thesis involves ...
PURIFICATION AND CHARACTERIZATION OF THE RECD PROTEIN-HOMOLOGUE FROM DEINOCOCCUS RADIODURANS
(2004-12-06)
In many gram-negative bacteria, RecBCD enzyme is found to be responsible for double strand DNA break repair through homologous recombination. The AddAB enzyme, a RecBCD analog, is found in some gram-positive bacteria and ...
DENSITY FUNCTIONAL CALCULATIONS OF BACKBONE 15N CHEMICAL SHIELDINGS IN PEPTIDES AND PROTEINS
(2009)
In this dissertation, we describe computational and theoretical study of backbone 15N chemical shieldings in peptides and proteins. Comprehensive density functional calculations have been performed on systems of different ...
Mass Spectrometric Analysis of Cytoplasmic Ribosomal Proteins in Drug Resistant and Drug Susceptible Human Cell Lines
(2006-05-30)
This study examines changes in cytoplasmic ribosomes that accompany drug resistance in MCF-7 breast cancer cells. Differences in ribosomal protein composition between drug susceptible and drug resistant cell lines were ...
Molecular Interactions of Ubiquitin and Polyubiquitin with Ubiquitin Binding Domains
(2007-10-22)
Ubiquitin is a small protein that is covalently attached to proteins, either as a single ubiquitin moiety or as polyubiquitin chains. A cascade of enzymatic reactions is required for the isopeptide linkage between the ...