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TURNIP CRINKLE VIRUS COAT PROTEIN EXPRESSION AND PURIFICATION
(2005-07-29)
Turnip crinkle virus is a (+) sense single strand RNA plant virus consisting of a single copy of the genome surrounded by 180 copies of the 38 kDa coat protein (CP) that comprise the virion capsid. As a structural protein, ...
SOLUTION ISOELECTRIC FOCUSING AND ITS APPLICATION IN COMPARATIVE PROTEOMIC STUDIES OF NUCLEAR PROTEINS
(2005-05-27)
In proteomic research, experimental and computational approaches are combined to provide global analysis of the entire proteomes of cells and tissues. The identification and quantification of multiple proteins, which ...
Structure of Fe isotopes at the limits of the pf shell
(2008-07-11)
An investigation of the structure of Fe isotopes up to neutron number 40 has been carried out at Argonne National Laboratory with the application of a thick-target deep-inelastic reaction experiment. A beam of 450 MeV <sup>64</sup>Ni was incident on a 55 mg/cm<sup>2</sup> target of enriched <sup>238</sup>U located at the center of the Gammasphere spectrometer. Triple coincidence data obtained from the experiment were sorted into four time-correlated cubes and used to identify new levels in <sup>61,62,64</sup>Fe. Angular correlations were used to both confirm previously-assigned spin and parity assignments and to assign values to excited states established in this work for the first time. The effect of g<sub>9/2</sub> neutron excitations on the structure of low-lying yrast states in these isotopes was studied with a comparison of experimental levels with the results from shell model calculations within both pf and truncated pfg configuration spaces. The effective interactions used in this work were derived from the N3LO nucleon-nucleon potential. These calculations indicate a strong influence from the g<sub>9/2</sub> orbital, beginning at moderate energy and spin in <sup>62</sup>Fe and extending to the low-lying states of <sup>64</sup>Fe. New levels identified above a 239-ns, 9/2<sup>+</sup> isomer in <sup>61</sup>Fe appear to be consistent within a rotation-aligned coupling scheme, with prolate deformation beta ~ 0.24, a value supported with both the shell model and Particle-triaxial rotor model. The data from this work mark a significant achievement in terms of understanding the evolution of nuclear structure in this region and the possible onset of deformation near N = 40. Still, more theoretical work is needed in order to better characterize experimentally observed features of this region. In addition to the Fe experiments described in the body of this thesis, another measurement was carried out in which the structure of <sup>128</sup>Cd was investigated. This nucleus, like <sup>64</sup>Fe, can be viewed as two proton and two neutron holes in a double-magic system. The identification of isomeric decay, and a confirmation of 2<sup>+</sup> and 4<sup>+</sup> level energies is described....
Molecular Structure and Surface Organization: A Study of Liquid/Vapor Interfaces Using Newly Developed Sum Frequency Methods
(2004-08-19)
Vibrational sum frequency spectroscopy (VSFS) is a surface specific nonlinear technique that provides vibrational spectra of molecules at interfaces. Studies presented in this thesis use VSFS to examine surface structure ...
Barcoded Silica Nanotubes for Bioanalysis
(2007-09-25)
Analysis of the chemical/biological species involved in health care is the most important step for diseases diagnosis and new drug screening. Barcoded nano/microparticles are attracting more and more interest for detection ...
Spatially Heterogeneous Dynamics and String-Like Correlated Motion in Supercooled Liquids and Polymers
(2004-04-16)
Dense liquids above their glass transition exhibit spatially heterogeneous dynamics (SHD) in which regions within the liquid exhibit enhanced or diminished mobility relative to the average on some time scale. The spatially ...
CAPILLARY ISOELECTRIC FOCUSING-BASED MULTIDIMENSIONAL PEPTIDE/PROTEIN SEPARATIONS FOR PROTEOMICS ANALYSIS
(2005-05-18)
With the completion of the human genome project, the proteomics has become the focus of research interest for better understanding the complex biological processes. The mass spectrometry (MS) detection of vast number and ...
Ethylene Polymerization Using a Zirconium Amidinate Supported Catalyst
(2004-05-07)
A series of W. R. Grace Davison IOLA(TM), methylaluminoxane-silica (MAO/Silica) and MAO/IOLA support materials were used to activate and immobilize a zirconium amidinate single site catalyst of the formula Cp*ZrMe2[tBuNC(Me)NCEt]. ...
Micelle and Aggregate Formation in Amphiphilic Block Copolymer Solutions
(2010)
The amphiphilic nature of many block copolymers causes self-aggregation and micelle formation in solvents that are miscible with only one of the block polymers (selective solvents). Micelle and aggregate formation of ...
CATANIONIC SURFACTANT VESICLES: TECHNOLOGY FOR VACCINE DEVELOPMENT AND TARGETED DRUG DELIVERY APPLICATIONS
(2013)
Catanionic surfactant vesicles have gained attention due to their structural similarities to liposomes and their robust properties in biological media. Catanionic vesicles are formed from oppositely charged surfactants ...