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Benzoyl-CoA reductase, isolated from the anaerobic bacterium Thauera aromatica, catalyzes the ATP-dependent, two-electron reduction of the aromatic ring of benzoyl-CoA. A Birch-like mechanism, which involves two separate ...

Peptide backbone cyclization is an important bio-engineering tool that has been widely used to control the stability, structure and biological role of small peptides. Here we have analyzed the effect of ligand binding on ...

The Escherichia coli chaperonin GroEL assists in the re-folding of misfolded substrate proteins (SPs). In response to the binding of ATP, GroEL undergoes large, allosteric structural transitions, resulting in an expansion ...

Lac repressor (LacI) when bound to two operator DNA sites loops the intervening DNA sequence, enhancing the efficiency of transcriptional repression. Previous results from footprinting, electrophoretic mobility shift and ...

A widely held view with respect to the folding of single-domain proteins is that they are two-state. In other words, it is seemingly sufficient to invoke just two thermodynamic macrostates - folded and unfolded - to explain ...

The MphR(A) transcriptional repressor protein was incorporated into high-efficiency ex-pression vectors, purified and characterized. Initial screens for crystallography solvents were conducted along with preliminary ...

Protein-induced bending of DNA plays an important role in regulating its transcription, replication, recombination, and packaging into nucleosomes. In particular, many general and gene-specific transcription factors bend ...

Besides the complexity of protein samples, probably the greatest challenge presently facing comprehensive proteome analysis is related to the large variation of protein relative abundances (>6 orders of magnitude), having ...

Thymine dimers are premutagenic lesions that form via ultraviolet irradiation of DNA. While the distribution of thymine dimers is non-random, it is also not yet predictable. Thymine dimer accumulation is likely to be ...

The need for iodide in biology is almost exclusively limited to its role in thyroid hormones, yet the recycling of thyroidal iodide is still critical for human health. The flavoprotein iodotyrosine deiodinase (IYD) salvages ...