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Now showing items 11-20 of 98
DESIGN AND SYNTHESIS OF AN ELECTRON RICH QUINONE METHIDE PRECURSOR FOR SEQUENCE -DIRECTED ALKYLATION OF DNA
(2012)
Quinone methides (QM) can be delivered to alklyate specific sites with a single strand DNA through target promoted alkylation. Previous experimental results indicated that alkylation by DNA-QM self-adduct was too slow for ...
Discovery, Characterization and Mechanistic Study of a Novel L-Tyrosine Hydroxylase in the Biosynthesis of Anthramycin
(2012)
A tyrosine hydroxylase coded by <italic>orf13</italic> of the anthramycin biosynthesis gene cluster is proposed to catalyze the <italic>ortho</italic>-hydroxylation of L-tyrosine to L-DOPA as the initial step of a unique ...
MANIPULATION OF DNA TOPOLOGY USING AN ARTIFICIAL DNA-LOOPING PROTEIN
(2012)
DNA loop formation, mediated by protein binding, plays a broad range of roles in cellular function from gene regulation to genome compaction. While DNA flexibility has been well investigated, there has been controversy in ...
Iodotyrosine Deiodinase from Selected Phyla Engineered for Bacterial Expression
(2012)
Iodide is a well known halogen necessary for development. The majority of iodide processing in biological systems occurs in the thyroid gland. Iodide salvage is essential to thyroid hormone metabolism and metabolic regulation. ...
THE DEVELOPMENT AND APPLICATION OF TRANSPOSON MUTAGENESIS AND UNNATURAL AMINO ACID MUTAGENESIS TECHNOLOGIES
(2012)
Recent three decades have seen a significant progress in the protein engineering field. Protein engineering has not only facilitated our understanding of the sequence-structure-function relationship of proteins, but also ...
SINGLE MOLECULE FRET OF LACI-DNA-IPTG LOOP CONFORMATIONS
(2012)
This work focuses on the <italic>Escherichia coli</italic> lactose repressor protein (LacI) which represses expression of the lac operon. In order to repress transcription, the tetrameric LacI protein binds a primary ...
Structural Studies of the Atypical Rio2 Kinase and N-Acetylglutamate Synthase
(2011)
Rio2 is required for small subunit ribosomal RNA (rRNA) maturation. It is specifically involved in site D cleavage of the 20S rRNA to produce the mature 18S rRNA. Loss of Rio2p is lethal and a decrease of Rio2p activity ...
USING SINGLE MOLECULE TECHNIQUES TO DETERMINE THE MECHANISM OF DNA TOPOLOGY SIMPLIFICATION BY TYPE IIA TOPOISOMERASES
(2011)
Type IIA topoisomerases are essential, universally conserved proteins that modify DNA topology by passing one segment of duplex DNA (the transfer, or T-segment) through a transient double strand break in a second segment ...
Differentiating a Landscape of Lac Repressor Mediated DNA Loops Using FRET
(2011)
The variety of DNA loops formed by a protein such as Lac Repressor (LacI), predicted to populate multiple loop topologies and geometries, increases the uncertainty of models that have not been experimentally verified. We ...
Structural Studies of Nep1/Emg1 RNA Methyltransferase and Atypical Rio3 Kinase
(2011)
Nucleolar Essential Protein 1 (Nep1) is required for small subunit (SSU) ribosomal RNA (rRNA) maturation and is mutated in Bowen-Conradi Syndrome. Although yeast (Saccharomyces cerevisiae) Nep1 interacts with a consensus ...