Now showing items 1-6 of 6
The Transcriptional Regulator MphR(A): Characterization and Circuit Engineering
The MphR(A) transcriptional repressor protein was incorporated into high-efficiency ex-pression vectors, purified and characterized. Initial screens for crystallography solvents were conducted along with preliminary ...
TARGETING BIOMARKERS VIA CITP-BASED SELECTIVE PROTEOME ENRICHMENT
Besides the complexity of protein samples, probably the greatest challenge presently facing comprehensive proteome analysis is related to the large variation of protein relative abundances (>6 orders of magnitude), having ...
Fundamentals of Excess Electron Transport in Biologically Relevant Systems
Thymine dimers are premutagenic lesions that form via ultraviolet irradiation of DNA. While the distribution of thymine dimers is non-random, it is also not yet predictable. Thymine dimer accumulation is likely to be ...
SINGLE MOLECULE FLUORESCENCE INVESTIGATION OF PROTEIN FOLDING
In addition to the well known two-state folding scenario, the energy landscape theory of protein folding predicts the possibility of downhill folding under native conditions. This intriguing prediction was extended by ...
Mutational Analysis of the Downhill Folding Protein gpW: Towards Tuning Stability of a Molecular Rheostat Candidate
A popular convention derived from early experimental evidence of single-domain proteins pointed towards a common mechanism of achieving their native three-dimensional structure. Concomitantly, protein function has been ...
Structural Determinants for Allostery and Functional Switching in the E. coli Biotin Regulatory System
The Escherichia coli protein, BirA, functions as an allosteric transcriptional repressor and a metabolic enzyme. BirA catalyzes synthesis of its intermediate, bio-5'-AMP, from biotin and ATP. In its repressor function, ...