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A Thermodynamic Investigation into the Allosteric Activation Mechanism of the Biotin Repressor
(2004-11-05)
The biotin regulatory system of Escherichia coli serves as a model for investigating the regulatory mechanism of a non-classical allosteric transcription factor. The central protein, BirA, functions as both an essential ...
NMR studies of polyubiquitin chains: insights into structural basis of functional diversity
(2004-10-25)
Signaling by polyubiquitin (polyUb) chains mediates numerous cellular processes. All these processes involve the covalent modification of the substrate protein with a polyubiquitin chain that is itself formed by isopeptide ...
Effect of ligand binding on the backbone dynamics of linear and circular constructs of SH3 Domain
(2004-12-08)
Peptide backbone cyclization is an important bio-engineering tool that has been widely used to control the stability, structure and biological role of small peptides. Here we have analyzed the effect of ligand binding on ...
Location specific recognition of the recombination hot-spot "Chi" by the E. coli RecBCD enzyme
(2004-04-06)
RecBCD is an ATP-dependent helicase and exonuclease, which generates 3'-single-stranded DNA (ssDNA) ends used by RecA for homologous recombination. Critical to this function is its encounter with an regulatory DNA octamer ...
PURIFICATION AND CHARACTERIZATION OF THE RECD PROTEIN-HOMOLOGUE FROM DEINOCOCCUS RADIODURANS
(2004-12-06)
In many gram-negative bacteria, RecBCD enzyme is found to be responsible for double strand DNA break repair through homologous recombination. The AddAB enzyme, a RecBCD analog, is found in some gram-positive bacteria and ...
Allostery and GroEL: Exploring the Tenets of Nested Cooperativity
(2004-06-24)
Despite a wealth of structural and biochemical studies on the functional cycle of the <i>E. coli</i> chaperonins GroEL and GroES, no model proposed to date accounts for all the effects seen experimentally by ...
Evaluation of Acetonitrile Precipitation as a Method for Separating Small from High Molecular Mass Proteins in Cytosol from MCF-7 Breast Cancer Cells.
(2004-05-18)
Acetonitrile precipitation has been carried out as an investigative method for separating small from high molecular mass proteins. The separation has been visualized on a 1-D gel electrophoresis. Acetonitrile helps drive ...
Proteomic Analysis of Plasma Membrane Proteins from Drug Susceptible and Drug Resistant Breast Cancer Cell Lines
(2004-11-08)
Drug discovery is an important field of research in the biotechnology and pharmaceutical industries. Plasma membranes are rich in drug targets and other proteins responsible for cell signaling, transport, signal transduction, ...