Search
Now showing items 1-10 of 22
PRINCIPLES OF COMPLEX POLYUBIQUITIN SIGNALING, THE STRUCTURAL BASIS FOR UBIQUITIN-UBISTATIN INTERACTIONS, AND NOVEL ASSAYS FOR THE CHARACTERIZATION OF DEUBIQUITINASES
(2013)
Ubiquitination is the most versatile and is certainly one of the most difficult post-translation modifications to understand in eukaryotic life. In the process of ubiquitination the C-terminus of ubiquitin (Ub), a small ...
THE DYNAMICS OF DIUBIQUITIN REVEALED BY NMR: WHAT IS THE DRIVING FORCE BETWEEN THE OPEN AND CLOSED STATES?
(2012)
The K48-linked polyubiquitin chains are important signals for proteasomal degradation and other biological processes. Their recognition of ubiquitin binding partners such as the UBA2 (ubiquitin associated domain 2) domain ...
CHARACTERIZATION OF BRANCHED HISTIDINE-LYSINE POLYPEPTIDES USED FOR NUCLEIC ACID DELIVERY AND THEIR COMPLEXES WITH DNA AND SMALL INTERFERING RNA.
(2013)
DNA and siRNA must be packaged for protection prior to transfection in vivo when they are administered to humans or other animals. Dr. A. J. Mixson's laboratory at the University of Maryland, Baltimore Medical School has ...
SINGLE MOLECULE FRET OF LACI-DNA-IPTG LOOP CONFORMATIONS
(2012)
This work focuses on the <italic>Escherichia coli</italic> lactose repressor protein (LacI) which represses expression of the lac operon. In order to repress transcription, the tetrameric LacI protein binds a primary ...
USING SINGLE MOLECULE TECHNIQUES TO DETERMINE THE MECHANISM OF DNA TOPOLOGY SIMPLIFICATION BY TYPE IIA TOPOISOMERASES
(2011)
Type IIA topoisomerases are essential, universally conserved proteins that modify DNA topology by passing one segment of duplex DNA (the transfer, or T-segment) through a transient double strand break in a second segment ...
Differentiating a Landscape of Lac Repressor Mediated DNA Loops Using FRET
(2011)
The variety of DNA loops formed by a protein such as Lac Repressor (LacI), predicted to populate multiple loop topologies and geometries, increases the uncertainty of models that have not been experimentally verified. We ...
BIOPHYSICAL STUDIES OF THE MECHANISM OF CERAMIDE CHANNEL DESTABLIZATION BY BCL-XL IN APOPTOSIS AND THE USE OF RECTIFICATION TO PROBE THE STRUCTURE AND DYNAMICS OF A NOVEL ESCHERICHIA COLI CHANNEL
(2015)
Ceramide forms a novel type of channel in the mitochondrial outer membrane and these channels are involved the release of intermembrane space proteins from mitochondria, a decision-making step in the apoptotic process. An ...
Probing Allosteric Communication Between Disordered Surfaces in a Protein
(2015)
Molecular mechanisms of protein allostery are not well understood, particularly in those systems that undergo disorder to order transitions upon activation. BirA, an E.coli metabolic enzyme and transcriptional repressor, ...
Experimental, Computational and, Theoretical Analysis of the GroEL/GroES Catalytic Cycle
(2014)
The GroEL/GroES system is among the best characterized molecular chaperonins, having been the focus of numerous studies since its discovery just over 40 years ago. There are several aspects of the chaperonin's catalytic ...
THE GATING OF THE BACTERIAL MECHANOSENSITIVE CHANNEL MSCS REFLECTS ITS FUNCTION AS A SENSOR OF BOTH CROWDING AND LATERAL PRESSURE AS WELL AS ITS ROLE IN OSMOREGULATION
(2014)
The mechanosensitive channel MscS is a ubiquitous bacterial membrane valve that opens by increased tension in the event of osmotic down-shock, releasing small internal osmolytes and thus preventing the cell from excessive ...