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The conformational landscape of RNA translational regulators and their potential as drug discovery targets
(2016)
RNA is an underutilized target for drug discovery. Once thought to be a passive carrier of genetic information, RNA is now known to play a critical role in essentially all aspects of biology including signaling, gene ...
Structural characterization of interaction of K11 and K63 mixed-linkage polyubiquitin chains with the tUIMs of epsin1
(2016)
Ubiquitylation or covalent attachment of ubiquitin (Ub) to a variety of substrate proteins in cells is a versatile post-translational modification involved in the regulation of numerous cellular processes. The distinct ...
Development of Magnetic Nanoparticle-based Enrichment Techniques and Mass Spectrometry Methods for Quantification of the Clinical Biomarker Cardiac Troponin I
(2016)
Human cardiac troponin I (cTnI) in serum is a well-known clinical biomarker for cardiac tissue damage and is used for diagnosing myocardial infarction. Unfortunately, commercial cTnI immunoassays from different manufacturers ...
DESIGN, SYNTHESIS, AND ANTIBODY BINDING STUDIES OF HIV-ASSOCIATED CARBOHYDRATE ANTIGENS
(2016)
The development of an effective prophylactic human immunodeficiency virus (HIV) vaccine is a critical global health priority. However, to-date, all efforts to design an HIV vaccine have been met with a paucity of success. ...
QUANTITATIVE ANALYSIS OF INTACT PROTEINS AND RNAS CARRIED BY IMMUNOSUPPRESSIVE EXOSOMES
(2016)
Myeloid-derived suppressor cells (MDSC) are immature myeloid cells that accumulate in the tumor microenvironment of most cancer patients. They are a major obstacle to immunotherapy because they suppress both adaptive and ...
THERMODYNAMICS AND APPLICATION OF A PAIR OF SYNTHETIC NUCLEOBASES FROM ARTIFICIALLY EXPANDED GENETIC INFORMATION SYSTEM
(2016)
UV-melting experiments were performed on 9-mer duplexes containing a pair of synthetic nucleobases P·Z, two members of Expanded Genetic Information System (AEGIS), or P, Z containing mismatches. Enthalpy, entropy and free ...
Intercepting Cyclic Dinucleotide Signaling with Small Molecules
(2016)
Bacterial infections, especially the ones that are caused by multidrug-resistant strains, are becoming increasingly difficult to treat and put enormous stress on healthcare systems. Recently President Obama announced a new ...
ENZYME-FREE UBIQUITIN LIGATION. FROM NATIVE CHEMICAL LIGATION AND SPIN LABELING TO DIMERIZATION BY INTER-UBIQUITIN MIMICKING LINKAGE AND PH-DEPENDENT CONFORMATIONAL SWITCH
(2016)
The delicate balance between the production and disposal of proteins is vital for the changes required in the cell to respond to given stimulus. Ubiquitination is a protein modification with a range of signaling outcomes ...
INVESTIGATION BY MASS SPECTROMETRY OF THE UBIQUITOME AND PROTEIN CARGO OF EXOSOMES DERIVED FROM MYELOID-DERIVED SUPPRESSOR CELLS
(2016)
Exosomes released by myeloid-derived suppressor cells (MDSC) are 30 nm in diameter extracellular vesicles that have been shown to carry biologically active proteins as well as ubiquitin molecules. Ubiquitin is known to ...
STRUCTURAL AND FUNCTIONAL STUDIES OF CYCLIC K48-LINKED DIUBIQUITIN
(2016)
K48-linked di-ubiquitin exists in a dynamic equilibrium between open and closed states. The structure of K48-Ub2 in the closed conformation features a hydrophobic interface formed between the two Ub domains. The same ...