Search

The K48-linked polyubiquitin chains are important signals for proteasomal degradation and other biological processes. Their recognition of ubiquitin binding partners such as the UBA2 (ubiquitin associated domain 2) domain ...

Acquired drug resistance is a multifactorial process that is one of the major causes for cancer treatment failure. The anticancer drug, mitoxantrone, was recently determined to inhibit ribosome biogenesis. Changes in ...

Our goal is to develop, optimize and demonstrate workflows that incorporate rapid Asp-selective chemical proteolysis into proteomic studies of complex mixtures. This can be further divided into several specific aims. The ...

Multifunctional proteins utilize several strategies to interact with different partners, resulting in diverse cellular outcomes. Structural, thermodynamic and kinetic features of these proteins influence the switch between ...

Quinone methides (QM) can be delivered to alklyate specific sites with a single strand DNA through target promoted alkylation. Previous experimental results indicated that alkylation by DNA-QM self-adduct was too slow for ...

A tyrosine hydroxylase coded by <italic>orf13</italic> of the anthramycin biosynthesis gene cluster is proposed to catalyze the <italic>ortho</italic>-hydroxylation of L-tyrosine to L-DOPA as the initial step of a unique ...

DNA loop formation, mediated by protein binding, plays a broad range of roles in cellular function from gene regulation to genome compaction. While DNA flexibility has been well investigated, there has been controversy in ...

Iodide is a well known halogen necessary for development. The majority of iodide processing in biological systems occurs in the thyroid gland. Iodide salvage is essential to thyroid hormone metabolism and metabolic regulation. ...

Recent three decades have seen a significant progress in the protein engineering field. Protein engineering has not only facilitated our understanding of the sequence-structure-function relationship of proteins, but also ...

This work focuses on the <italic>Escherichia coli</italic> lactose repressor protein (LacI) which represses expression of the lac operon. In order to repress transcription, the tetrameric LacI protein binds a primary ...