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Dynamics and Chemical Shielding in a Small Protein from NMR Spin Relaxation Experiments
(2006-08-04)
NMR spin relaxation spectroscopy is a proven method for characterization of the time-scales and amplitudes of intramolecular motions in proteins. In a typical NMR relaxation experiment, the significant contributions to ...
EXPRESSING DEINOCOCCUS RADIODURANS RECD IN ESCHERICHIA COLI: PHENOTYPIC EFFECTS IN RECBCD(-) AND RECD(-) CELLS
(2006-12-20)
The RecD helicase is a member of the RecBCD complex, which is essential for repair of double-stranded breaks (DSBs) in DNA via homologous recombination in Escherichia coli. The microbe Deinococcus radiodurans is capable ...
Conformational And Assembly Studies Of Two Tau Repeats: 2R and 3R
(2006-12-19)
Our aim was to investigate the conformational properties of two tau fragments, 2R and 3R, in response to increasing amounts of a hydrogen-bond stabilizing solvent trifluoroethanol. 2R and 3R contain two or three of the ...
Insight into the Structure and Mechanism of Iodotyrosine Deiodinase, the First Mammalian Member of the NADH Oxidase / Flavin Reductase Superfamily
(2006-07-18)
Iodotyrosine deiodinase (IYD) has remained poorly characterized for nearly 50 years in spite of its function as an iodine salvage pathway and its role in regulation of mammalian basal metabolism. IYD catalyzes the reductive ...
Mass Spectrometric Analysis of Cytoplasmic Ribosomal Proteins in Drug Resistant and Drug Susceptible Human Cell Lines
(2006-05-30)
This study examines changes in cytoplasmic ribosomes that accompany drug resistance in MCF-7 breast cancer cells. Differences in ribosomal protein composition between drug susceptible and drug resistant cell lines were ...
Rapid kinetics study of the nuclease activity of RecBCD enzyme from Escherichia coli
(2006-03-31)
RecBCD enzyme from Escherichia coli is involved in homologous recombination and repair of bacterial DNA, and in defending the cell against foreign DNA. This enzyme has multiple functions - it is an ATP-stimulated ssDNA ...
MAPPING OF METAL ION BINDING SITES IN THE RECBCD ENZYME AND THE ROLE OF MAGNESIUM IN THE SUBUNIT INTERACTIONS OF RECBCD
(2006-01-24)
E. coli RecBCD is a 330kDa enzyme with three subunits RecB, RecC and RecD. The biological functions of RecBCD are to degrade foreign linear DNA and provide single stranded DNA to initiate homologous recombination. The ...