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The Development of New Tools for the Investigation of Protein Function Using Photo-Reactive Unnatural Amino Acids
Reported here is the direct synthesis and application of unnatural amino acids for the development of exploratory tools for protein studies. This work takes advantage of an expanded genetic code to extract a more precise ...
Top-Down Analysis of Bacterial Proteins by High-Resolution Mass Spectrometry
In the biodefense and medical diagnostic fields, MALDI mass spectrometry-based systems are used for rapid characterization of microorganisms generally by detecting and discriminating the highly abundant protein mass-to-charge ...
THERMODYNAMIC PROPERTIES OF THE UNFOLDED ENSEMBLE OF PROTEINS
A random coil, whose size is determined by its excluded volume, and net energetic interactions with its environment, has served as a representation of the unfolded ensemble of proteins. The work in this thesis involves ...
Kinetic Characterization and Domain Analysis of the helicase RecD2 from Deinococcus radiodurans
The gram positive bacterium <italic>D. radiodurans</italic> is known for its extreme resistance to radiation and an extraordinary ability to reconstitute its genome after sustaining large numbers of double strand breaks ...
Kinetic and Structual Characterization of Glutamine-Dependent NAD Synthetases
Multifunctional enzymes catalyzing successive reactions have evolved several mechanisms for the transport of intermediates between active sites. One mechanism, substrate channeling, allows the transport of the intermediate ...