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NMR studies of polyubiquitin chains: insights into structural basis of functional diversity
(2004-10-25)
Signaling by polyubiquitin (polyUb) chains mediates numerous cellular processes. All these processes involve the covalent modification of the substrate protein with a polyubiquitin chain that is itself formed by isopeptide ...
Applications of Multiphoton Imaging Techniques To The Study Of Protein Interactions
(2009)
Several recent improvements in microscopy have been driven by advances in ultrafast laser technology. The goal of the research described in this dissertation was to develop noninvasive, optically based methods to measure ...
Fluorescence and NMR Studies of the Role of Metal Ions in HIV-1 Genomic RNA Dimerization and Maturation
(2009)
The dimerization initiation site (DIS) is an essential RNA element responsible for dimerization of HIV-1 genomic RNA through a kissing loop interaction. The DIS loop contains six auto-complementary nucleotides stabilized ...
One-Dimensional Free Energy Surface Models of Protein Folding: Connecting Theory and Experiments
(2007-04-27)
Experimental techniques have now reached the sub-microsecond timescale necessary to study fast events in protein folding. However, analysis of fast folding experiments still commonly rely on conventional procedures that ...
Stretching Biomolecules
(2005-11-23)
Biomolecular self-assembly is the complicated processes characterized by broad and hierarchical structure of energy, length, and time scales.
Various experimental tools have, for decades, been used to understand the ...
Protein folding and amyloid formation in various environments
(2008-11-21)
Understanding and predicting the effect of various environments that differ in terms of pH and the presence of cosolutes and macromolecules on protein properties is a formidable challenge. Yet this knowledge is crucial in ...
CERAMIDE PERMEABILIZATION OF MITOCHONDRIAL OUTER MEMBRANE: PHARMACOLOGICAL CHARACTERIZATION AND RELATION TO MAC AND BAX
(2005-12-08)
In apoptosis, the mitochondrial outer membrane (MOM) becomes permeable, releasing proteins. This permeability has been attributed to the action of various factors, including mitochondrial apoptosis-induced channel (MAC), ...
Barrier Heights and Diffusion Coefficients in Protein Folding
(2007-08-03)
A widely held view with respect to the folding of single-domain proteins is that they are two-state. In other words, it is seemingly sufficient to invoke just two thermodynamic macrostates - folded and unfolded - to explain ...
Mutational Analysis of the Downhill Folding Protein gpW: Towards Tuning Stability of a Molecular Rheostat Candidate
(2008-09-24)
A popular convention derived from early experimental evidence of single-domain proteins pointed towards a common mechanism of achieving their native three-dimensional structure. Concomitantly, protein function has been ...
Dendritic Integration and Reciprocal Inhibition in the Retina
(2008-11-17)
The mammalian retina is capable of signaling over a vast range of mean light levels (~10^10). Such a large dynamic range is achieved by segregating signals into contrasting pathways and utilizing excitatory and inhibitory ...