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A Thermodynamic Investigation into the Allosteric Activation Mechanism of the Biotin Repressor
(2004-11-05)
The biotin regulatory system of Escherichia coli serves as a model for investigating the regulatory mechanism of a non-classical allosteric transcription factor. The central protein, BirA, functions as both an essential ...
Dynamics and Chemical Shielding in a Small Protein from NMR Spin Relaxation Experiments
(2006-08-04)
NMR spin relaxation spectroscopy is a proven method for characterization of the time-scales and amplitudes of intramolecular motions in proteins. In a typical NMR relaxation experiment, the significant contributions to ...
NMR studies of polyubiquitin chains: insights into structural basis of functional diversity
(2004-10-25)
Signaling by polyubiquitin (polyUb) chains mediates numerous cellular processes. All these processes involve the covalent modification of the substrate protein with a polyubiquitin chain that is itself formed by isopeptide ...
Investigation into the Driving Force Dependence of Excess Electron Transport in Duplex DNA
(2009)
The purpose of this dissertation is to investigate the driving force dependence of excess electron transport in deoxyribonucleic acid (DNA) using napthyl amines as electron donors.
The ability of DNA to act as a pathway ...
TURNIP CRINKLE VIRUS COAT PROTEIN EXPRESSION AND PURIFICATION
(2005-07-29)
Turnip crinkle virus is a (+) sense single strand RNA plant virus consisting of a single copy of the genome surrounded by 180 copies of the 38 kDa coat protein (CP) that comprise the virion capsid. As a structural protein, ...
The Development of New Tools for the Investigation of Protein Function Using Photo-Reactive Unnatural Amino Acids
(2010)
Reported here is the direct synthesis and application of unnatural amino acids for the development of exploratory tools for protein studies. This work takes advantage of an expanded genetic code to extract a more precise ...
SITE-DIRECTED MUTAGENESIS OF GROEL:DEVELOPING A SYSTEM FOR MONITORING ALLOSTERIC MOVEMENTS BY FLUORESCENCE RESONANCE ENERGY TRANSFER
(2007-01-02)
The Escherichia coli chaperonin protein GroEL can assist protein folding to its native state through the consumption of ATP. Accompanying this process, GroEL undergoes structural change, resulting in an expansion of the ...
EXPRESSING DEINOCOCCUS RADIODURANS RECD IN ESCHERICHIA COLI: PHENOTYPIC EFFECTS IN RECBCD(-) AND RECD(-) CELLS
(2006-12-20)
The RecD helicase is a member of the RecBCD complex, which is essential for repair of double-stranded breaks (DSBs) in DNA via homologous recombination in Escherichia coli. The microbe Deinococcus radiodurans is capable ...
One-Dimensional Free Energy Surface Models of Protein Folding: Connecting Theory and Experiments
(2007-04-27)
Experimental techniques have now reached the sub-microsecond timescale necessary to study fast events in protein folding. However, analysis of fast folding experiments still commonly rely on conventional procedures that ...
Conformational And Assembly Studies Of Two Tau Repeats: 2R and 3R
(2006-12-19)
Our aim was to investigate the conformational properties of two tau fragments, 2R and 3R, in response to increasing amounts of a hydrogen-bond stabilizing solvent trifluoroethanol. 2R and 3R contain two or three of the ...