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dc.contributor.authorBaykousheva, S. P.
dc.contributor.authorLuthria, D. L.
dc.contributor.authorSprecher, H.
dc.date.accessioned2011-07-05T11:44:50Z
dc.date.available2011-07-05T11:44:50Z
dc.date.issued1994
dc.identifier.citationBaykousheva, S. P.; Luthria, D. L.; Sprecher, H., Arachidonic acid formed by peroxisomal β-oxidation of 7,10,13,16- docosatetraenoic acid is esterified into 1-acyl-sn-glycero-3-phosphocholine by microsomes. Journal of Biological Chemistry 1994, 269 (28), 18390-18394.en_US
dc.identifier.issn0021-9258
dc.identifier.otherhttp://www.jbc.org/content/269/28/18390.full.pdf+html
dc.identifier.urihttp://hdl.handle.net/1903/11419
dc.descriptionAvailable online at http://www.jbc.org/content/269/28/18390.full.pdf+htmlen_US
dc.description.abstractPeroxisomal beta-oxidation of linoleic acid and arachidonic acid was depressed when 1-palmitoyl-sn-glycero-3-phosphocholine and microsomes were included in incubations. This reduction was due to the esterification of the substrate into the acceptor by microsomal 1-acyl-sn-glycero-3- phosphocholine acyltransferase. The first cycle of the beta-oxidation of 7,10,13,16-docosatetraenoic acid was independent of 1-acyl-sn-glycero-3-phosphocholine and microsomes. However, when arachidonate was produced it was esterified rather than serving as a substrate for continued beta-oxidation. When arachidonate and linoleate were incubated with peroxisomes alone, 2-trans-4,7,10-hexadecatetraenoic acid and 2-trans-4-decadienoic acid were the respective end products of beta-oxidation. 2-Oxo-8,11-heptadecadienone, a catabolite produced from linoleate, was most likely a nonenzymatic decarboxylation product of 3-oxo-9,12-octadecadienoic acid. In addition to the termination of beta-oxidation by microsomal-peroxisomal communication, our results with linoleate and arachidonate suggest that the reaction catalyzed by 2-trans-4-cis-dienoyl-CoA reductase is the control step in double bond removal. In addition, the beta-ketothiolase step may play a regulatory role in the peroxisomal beta-oxidation of linoleate but not arachidonate or 7,10,13,16-docosatetraenoic acid.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc.en_US
dc.subjectBaykoushevaen_US
dc.subjectBaykouchevaen_US
dc.subjectarachidonic aciden_US
dc.subjectMass spectrometryen_US
dc.subjectMicrosomesen_US
dc.subjectLiveren_US
dc.subjectRatsen_US
dc.subjectMetabolismen_US
dc.titleArachidonic acid formed by peroxisomal β-oxidation of 7,10,13,16- docosatetraenoic acid is esterified into 1-acyl-sn-glycero-3-phosphocholine by microsomesen_US
dc.typeArticleen_US
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland Librariesen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us


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